菜用大豆耐低温弱光鉴定方法和评价指标的研究

为建立一套有效、可靠的菜用大豆耐低温弱光鉴定方法和评价指标，选择12个熟性不同的春播型菜用大豆品种材料，通过对其在低温弱光胁迫下种子发芽快慢、幼苗存活率及相关生理生化指标等的检测分析，鉴定各品种材料对低温弱光逆境胁迫反应的差异。结果表明，在弱光低温组合60~80 μmol/(m2?s)、12℃的逆境胁迫下，12个菜用大豆品种材料的出苗快慢、幼苗存活率及叶片中SOD、POD及CAT活性等存在差异，相关分析表明保护性酶活性与光温组合呈极显著正相关。

Study on Index of Evaluation and Method of Identification in Low Temperature and Weak Light Tolerance of Vegetable Soybean

To establish an effective, reliable identification method and evaluation index of soybean resistance to low temperature and weak light, twelve vegetable soybean strains were primary studied on the difference of adaptability to low temperature and weak light with germination time, survival ratio, index of biochemistry and morphology. The results were proved germination time, survival ratio, active of SOD, POD and CAT of vegetable soybean existed evident differences under the condition of light （60-80 μmol/（m 2 ·s））, temperature （12℃）, and relevant analysis showed that the protective enzyme activity and light temperature combination was very significant positive correlation.