| 改良CTAB法快速提取棉花DNA |
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| 引用本文： | 宋国立,崔荣霞,王坤波,郭立平,黎绍惠,王春英,张香娣.改良CTAB法快速提取棉花DNA[J].棉花学报,1998(5). |
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| 作者姓名： | 宋国立 崔荣霞 王坤波 郭立平 黎绍惠 王春英 张香娣 |
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| 作者单位： | 中国农科院棉花研究所 |
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| 摘 要： | 针对棉花（Ｇｏｓｓｐｉｕｍ）富含棉酚、多糖等其它次生干扰物质这一特点而设计的一种快速分离和纯化棉花总ＤＮＡ（ｇＤＮＡ）的方法。该方法是对ＣＴＡＢ（ｃｅｔｙｌｔｒｉｍｅｔｈｙｌａｍｍｏｎｉｕｍｂｒｏｍｉｄｅ）法的改良，它在ＣＴＡＢ法的基础上，首先用ＤＩＥＣＡ（ｄｉｅｔｈｙｌｄｉｔｈｉｏｃａｒｂａｍｉｃａｃｉｄ）抑制酚氧化酶的活性，然后用活性炭和ＰＶＰ４０（ｐｏｌｙｖｉｎｙｌｐｙｒｒｏｌｉｄｏｎｅ）排除棉酚等其它次生干扰物质。试验证明，该方法比较简便、快速、经济、有效，得到的ｇＤＮＡ完全可以满足ＰＣＲ等分子生物学分析。
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| 关 键 词： | CTAB法 提取 棉花gDNA |
A Rapid Improved CTAB Method for Extraction of Cotton Genomic DNA |
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| Song guoli Cui rongxia Wang Kunbo Guo Liping Li Shaohui Wang Chunying Zhang Xiangdi Cotton Research Institute,CAAS,Key Laboratory of Cotton Genetical Improvement,Ministry of Agricalture of P. R. China,Anyang.A Rapid Improved CTAB Method for Extraction of Cotton Genomic DNA[J].Cotton Science,1998(5). |
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| Authors: | Song guoli Cui rongxia Wang Kunbo Guo Liping Li Shaohui Wang Chunying Zhang Xiangdi Cotton Research Institute CAAS Key Laboratory of Cotton Genetical Improvement Ministry of Agricalture of P R China Anyang |
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| Institution: | Song guoli Cui rongxia Wang Kunbo Guo Liping Li Shaohui Wang Chunying Zhang Xiangdi Cotton Research Institute,CAAS,Key Laboratory of Cotton Genetical Improvement,Ministry of Agricalture of P. R. China,Anyang 455112 |
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| Abstract: | This paper reported an improved CTAB(cetyltrimethyl ammonium bromide) method for rapid extraction cotton genomic DNA.It was used DIECA(diethyldithiocarbamic acid) to inactivate phenol oxidase ,and then added activated charcoal and PVP 40 (polyvinylpyrrolidone)to exclude polyphenol and other secondary interfacing substance.It showed that the resulting DNA is suitable for RAPD and other molecular biology analysis. |
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| Keywords: | CTAB Method Extraction cotton gDNA |
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