甘草离体培养物中总黄酮提取工艺的优化

【研究目的】对甘草离体培养物中总黄酮提取和测定工艺进行优化,以建立在室内筛选高产细胞系和试管苗株系的方法;【方法】采用超声波提取技术,对提取液、浸泡时间、提取温度等进行优化;【结果】将样品以75%甲醇浸泡6h后在32℃下超声提取两次,比色法测定总黄酮含量,加样回收率为95.3%～101.4%,相对标准偏差为1.61%。测得甘草初代愈伤组织中总黄酮含量为0.0012%～0.05%,子叶愈伤组织调控后含量高达0.08%,试管苗中总黄酮含量为1.04%～1.31%,移栽后高达4.5%。【结论】此工艺准确度较高、稳定性好、简便可行,为优良细胞系的筛选和试管苗的早期鉴定奠定了技术基础。

Extraction and Content Determination of Total Flavonoids of Cultures of Glycyrrhiza uralensis Fisch.

objective] to optimize the extraction and determination method of the total flavonoids thus to screen the cell clones and plantlets with high contents,method] the ultrasonic technique was used and several treatments were tried and screened,result] the best condition was marinated 6 hours with 75% carbinol then ultrasonic extracted twice.And then the content was determined with colorimetry from cultures of Glycyrrhiza uralensis Fisch.The result showed the recovery rate was between 95.3% and 101.4% and the RSD was 1.61%.The total flavonoids of the initial calli was between 0.0012% and 0.05%,the improvement cotyledonary calli was up to 0.08%,the contents of the plantlets were between 1.04% and 1.31%,and after being transplanted was up to 4.5%.conclusion] The rate of veracity of this method was high and this provided the determination of Glycyrrhiza cultures flavonoids products a feasible method,and those data offered a credible academic gist for authenticate of the experiment resource.