| 南极磷虾油中磷脂型脂肪酸的测定方法优化及应用 |
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| 引用本文： | 王诗淼,杨春雨,赵琪,李健.南极磷虾油中磷脂型脂肪酸的测定方法优化及应用[J].水产学报,2022,46(3):439-444. |
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| 作者姓名： | 王诗淼 杨春雨 赵琪 李健 |
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| 作者单位： | 大连工业大学食品学院 国家海洋食品工程技术研究中心,大连工业大学食品学院 国家海洋食品工程技术研究中心,大连工业大学食品学院 国家海洋食品工程技术研究中心,大连工业大学食品学院 国家海洋食品工程技术研究中心 |
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| 基金项目： | 国家重点研发计划(2018YFC1406805) |
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| 摘 要： | 南极磷虾营养价值高,因富含必需氨基酸、磷脂、虾青素和多不饱和脂肪酸(PUFAs)而具有良好的开发价值.目前,对南极磷虾及其制品脂肪酸含量的分析主要集中在总脂肪酸含量的测定,由于PUFAs不同酯的结合会影响其生物利用度,因此,有必要对南极磷虾不同类型酯的脂肪酸含量进行准确定量.为了明确南极磷虾油中磷脂(PL)型脂肪酸含量...
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| 关 键 词： | 南极磷虾 磷脂 多不饱和脂肪酸 固相萃取 气相色谱 |
| 收稿时间： | 2021/11/30 0:00:00 |
| 修稿时间： | 2022/2/12 0:00:00 |
Optimization and application of the method for determination of the fatty acid content in phospholipid from krill oil |
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| WANG Shimiao,YANG Chunyu,ZHAO Qi,LI Jian.Optimization and application of the method for determination of the fatty acid content in phospholipid from krill oil[J].Journal of Fisheries of China,2022,46(3):439-444. |
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| Authors: | WANG Shimiao YANG Chunyu ZHAO Qi LI Jian |
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| Institution: | National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University |
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| Abstract: | Due to rich in essential amino acids, phospholipids, astaxanthin and omega-3 polyunsaturated fatty acids (PUFAs), Antarctic krill (Euphausia superba) which was abundant and had high nutritional value was considered to have great development value. At present, the analysis of fatty acid contents in Antarctic krill and its products mainly focused on the determination of total fatty acid contents. Owing to the effect of bioavailability for PUFAs in different esters, it was necessary to accurately quantify the content of fatty acid in different types of esters in Antarctic krill and its products. In order to clarify the content of phospholipids (PL) fatty acids in Antarctic krill oil, a gas chromatography analysis method for the content of fatty acids in PL in Antarctic krill oil was established based on solid phase extraction. After optimizing the various conditions of the impact analysis, under the optimal conditions, the recoveries of the standard addition measured by this method were between 84.22%-94.45%, and the intra-day and inter-day precision were between 0.79%-6.09%. This method was applied to measure the changes in the content of various PL fatty acids of Antarctic krill oil during storage. The results showed that the content of PL fatty acids decreased during the 21-day storage process, and the degradation rate increases with increasing temperature. The present study provided methodological support for the study of lipids in Antarctic krill. |
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| Keywords: | Antarctic krill phospholipids polyunsaturated fatty acids solid phase extraction gas chromatography |
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