Aspects of fusion combining ability of dihaploidSolanum tuberosum L.: influence of the cytoplasm |
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Authors: | Ursula Frei Marietta Stattmann A Lössl G Wenzel |
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Institution: | (1) TU München-Weihenstephan, Hochanger 2, 85350 Freising, Germany;(2) Bioplant GmbH, 29574 Ebstorf, Germany |
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Abstract: | Summary Creation from 4x hybrid clones from protoplast fusion of 2x clones of potato was evaluated. Besides combined nuclear genomes,
composition of the cytoplasm significantly influenced the phenotypic traits of hybrid clones. To ascertain the influence of
parental cytoplasm on the success of protoplast fusion and regeneration of hybrid plants, data from 74 fusion combinations
of 50 dihaploid clones were analyzed. The majority of dihaploid breeding clones belonged to the cytoplasm types Wα, Tβ and
Wγ.
When the closely related mt types α, β and γ were used, fusion combinations had a better combining ability compared with more
distantly related cytoplasms δ and ⃛. Fusions containing the same mitochondrial type (homofusions) were not superior to closely
related mitochondrial types. However, homofusions of cytoplasm type Wα yielded significantly more hybrids than homofusions
of type Tβ. In general, parental cytoplasm types had little impact on the fusion combining behaviour. Thus the cytoplasm type
of the fusion parents is not a suitable marker for predicting the combining ability in protoplast fusion experiments. |
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Keywords: | potato mitochondria chloroplast protoplast fusion somatic hybridization cytoplasmic inheritance |
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