| 绵羊Granulysin基因的克隆与序列分析 |
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| 引用本文： | 乔新安,杨国宇,朱彦彩,王月影,韩立强,王艳玲. 绵羊Granulysin基因的克隆与序列分析[J]. 华中农业大学学报, 2008, 27(3) |
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| 作者姓名： | 乔新安 杨国宇 朱彦彩 王月影 韩立强 王艳玲 |
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| 作者单位： | 1. 河南农业大学牧医工程学院,郑州,450002
2. 河南科技学院动物科学学院,新乡,453003 |
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| 摘 要： | 基于电子延伸序列,设计1对克隆引物,从绵羊回肠黏膜组织中提取总RNA,进行RT-PCR,将PCR产物与pMD19-T载体连接后转化E. coli JM109感受态细胞、检测阳性克隆、测序并进行序列分析.克隆的绵羊Granulysin基因与人、牛该基因的同源性分别为56.2%和87.2%,推导的氨基酸序列信号肽为1～22氨基酸,SapB结构域为64～138氨基酸,结构特征与人、牛的一致.结果提示,绵羊Granulysin基因克隆成功.
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| 关 键 词： | 绵羊 颗粒溶解素 克隆 序列分析 |
Analysis on Molecular Cloning and Sequence of Ovine Granulysin |
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| QIAO Xi-nan,YANG Guo-yu,ZHU Yan-cai,WANG Yue-ying,HAN Li-qiang,WANG Yan-ling. Analysis on Molecular Cloning and Sequence of Ovine Granulysin[J]. Journal of Huazhong Agricultural University, 2008, 27(3) |
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| Authors: | QIAO Xi-nan YANG Guo-yu ZHU Yan-cai WANG Yue-ying HAN Li-qiang WANG Yan-ling |
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| Abstract: | A pair of cloning primers were designed based on the in silico sequence information.Total RNA was extracted from mucosal tissue of ovine ileum and mRNA sequence was amplified by RT-PCR.The PCR products were ligated into the pMD19-T vector,and then transformed into competent cells of E.coli JM109.The positive clone was identified and the sequence was sequenced and analysed.The results showed that ovine Granulysin shared 56.2% and 87.2% homology with that of human and cattle.The signal peptide of deduced prot... |
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| Keywords: | sheep Granulysin molecular cloning sequence analysis |
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