| 血清9型鸭疫里默氏杆菌在华南地区的分离鉴定 |
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| 引用本文： | 冯金牛,陈芳艳,区德庆,阮二垒,杨丽云,黎丁滔,陈瑞爱,唐秀英,王林川.血清9型鸭疫里默氏杆菌在华南地区的分离鉴定[J].中国预防兽医学报,2010,32(12). |
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| 作者姓名： | 冯金牛 陈芳艳 区德庆 阮二垒 杨丽云 黎丁滔 陈瑞爱 唐秀英 王林川 |
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| 作者单位： | 华南农业大学兽医学院;华南农业大学动物科学学院;广东省大华农动物保健品股份有限公司; |
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| 摘 要： | 本研究于2009年7月～2010年4月针对广东、广西、福建等养鸭密集地区进行鸭疫里默氏杆菌(RA)流行病学调查,并分离到5个RA分离株。经形态特征、生化特性和血清学鉴定表明,5个分离株与血清9型RA相似。对16S rRNA部分基因进行序列测定,并与GenBank中登录的菌株16S rRNA基因序列进行比对及系统进化分析显示:该5株分离株与报道的RA H-1785、H-2199、H-2565和P-2123的16S rRNA序列相似性较高,同源性达99.1%～99.7%,进一步证实这5个分离株为血清9型RA。
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| 关 键 词： | 鸭疫里默氏杆菌 血清型 系统进化分析 |
Isolation and identification of serotype 9 Riemerella anatipestifer in South China |
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| FENG Jin-niu,CHEN Fang-yan,OU De-qing,RUAN Er-lei,YANG Li-yun,LI Ding-tao,CHEN Ri-ai,TANG Xiu-ying,WANG Lin-chuan.Isolation and identification of serotype 9 Riemerella anatipestifer in South China[J].Chinese Journal of Preventive Veterinary Medicine,2010,32(12). |
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| Authors: | FENG Jin-niu CHEN Fang-yan OU De-qing RUAN Er-lei YANG Li-yun LI Ding-tao CHEN Ri-ai TANG Xiu-ying WANG Lin-chuan |
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| Institution: | FENG Jin-niu1#,CHEN Fang-yan3#,OU De-qing2,RUAN Er-lei1,YANG Li-yun1,LI Ding-tao1,CHEN Ri-ai2,TANG Xiu-ying2,WANG Lin-chuan1,2*(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China,2.Guangdong Dahuanong Animal Health Products Co.Ltd,Xinxing 527400,3.College of Animal Science,China) |
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| Abstract: | Five bacteria were isolated from Guangdong,Guangxi and Fujian during a Riemerella anatipestifer(RA) epidemiological investigation from July 2009 to April 2010 and identified as RA new serotypes(D10,D21,X18,J9 and J33),These isolates were similar to serotype 9 based on morphological characteristics,biochemical characteristics and serological identification.The 16S rRNA genes were sequenced and compared with those of related strains in GenBank.Phylogenetic analysis showed that isolates had 99.1% to 99.7% simi... |
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| Keywords: | Riemerella anatipestifer serotype phylogenetic analysis |
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