| 葡萄砧木‘F-242’组织培养及快繁技术 |
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| 引用本文： | 刘伟,李希东,刘新.葡萄砧木‘F-242’组织培养及快繁技术[J].北方园艺,2010(1):68-70. |
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| 作者姓名： | 刘伟 李希东 刘新 |
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| 作者单位： | 青岛农业大学,生命科学学院,山东,青岛,266109;青岛农业大学,生命科学学院,山东,青岛,266109;青岛农业大学,生命科学学院,山东,青岛,266109 |
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| 基金项目： | 农业部948资助项目(2006-G26) |
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| 摘 要： | 以葡萄砧木品种‘F-242’带芽新梢为供试材料,研究了消毒时间、激素种类及其浓度配比、基本培养基对外植体生根的影响。结果表明:75%乙醇15 s,0.1%的升汞8 min为葡萄砧木品种‘F-242’最佳的外植体消毒时间。附加生长素类物质IAA和IBA可以明显促进‘F-242’外植体的生根,且IAA处理的综合效果要明显优于IBAI、AA的最佳附加浓度为0.1 mg/L。不同的基本培养基对葡萄品种‘F-242’外植体生根影响显著,其中以MS基本培养基为最佳。
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| 关 键 词： | 葡萄 ’F-242’ 外植体 生根率 |
Establishment of Tissue Culture and Rapid Propagation System of Grape Rootstock 'F-242' |
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| LIU Wei,LI Xi-dong,LIU Xin.Establishment of Tissue Culture and Rapid Propagation System of Grape Rootstock 'F-242'[J].Northern Horticulture,2010(1):68-70. |
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| Authors: | LIU Wei LI Xi-dong LIU Xin |
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| Institution: | College of Life Sciences;Qingdao Agricultural University;Qingdao;Shansong 266109 |
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| Abstract: | In order to establish the efficient rapid propagation system of grape rootstock 'F-242',the effects of different disinfection times,hormones and their concentration ratio,basic mediums on rooting of explants were studied.The results indicated that the influence of different disinfection times were significantly different.The explants were sanitized in 75% alcohol for 15 s and then treated in 0.1% mercuric chloride for about 8 min,contamination can be controlled efficiently.The treatment which adding IAA and... |
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| Keywords: | grape 'F-242' explants rooting rate |
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