| 多花相思的组织培养和快速繁殖 |
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| 引用本文： | 张祖荣. 多花相思的组织培养和快速繁殖[J]. 福建林业科技, 2007, 34(2): 143-145 |
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| 作者姓名： | 张祖荣 |
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| 作者单位： | 重庆文理学院生命科学系,重庆,永川,402168 |
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| 基金项目： | 重庆市教委科研资助项目(渝教科200404)《三峡库区观赏植物资源调查及快繁技术研究》的部分内容 |
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| 摘 要： | 用多花相思茎段作外植体,采用在木本植物茎叶组培材料上经常采用的3种消毒方法,以及前人已在相思类植物营养器官组培上取得成功的4种芽诱导培养基、芽增殖培养基和生根培养基进行对比试验。结果表明,材料消毒以1/800灭菌净10 min+75%酒精50 s+0.1%HgCl25 min处理效果最好;芽诱导和芽增殖都以改良MS+6-BA1.0+NAA0.2效果最好;生根效果最好的培养基是1/2MS+IBA1.0。
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| 关 键 词： | 多花相思 茎段 组织培养 快速繁殖 |
| 文章编号： | 1002-7351（2007）02-0143-03 |
| 修稿时间： | 2006-11-16 |
The study on techniques for rapid propagation and tissue culture of Acacia floribunda (Vent.) Willd. |
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| ZHANG Zu-rong. The study on techniques for rapid propagation and tissue culture of Acacia floribunda (Vent.) Willd.[J]. Journal of Fujian Forestry Science and Technology, 2007, 34(2): 143-145 |
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| Authors: | ZHANG Zu-rong |
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| Affiliation: | Department of Life Science, Chongqing University of Arts and Sciences, Yongchuan, Chongqing 402168, China |
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| Abstract: | In this study,stem segments with axillary buds of Acacia floribunda were used as explants in tissue culture.Three common disinfect methods used in the tissue culture of wooden plant stems and leaves were adopted,and four culture media for bud induction,bud proliferation and rooting used in previous studies of the tissue culture of Acacia species were taken as a comparative tests.The result showed that the most effective method to disinfect material was 1/800 germicide for 10 min 75% alcohol for 50 s 0.1% HgCl2 for 5 min.The induced and proliferation media was the improved MS 6-BA1.0 NAA0.2,and the rooting was 1/2 MS IBA1.0. |
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| Keywords: | Acacia floribunda(Vent.) Willd. stem segment tissue culture rapid propagation |
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