| 低温预处理对中间偃麦草×普通小麦F_1幼穗愈伤组织诱导和继代培养的影响 |
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| 引用本文： | 伍碧华. 低温预处理对中间偃麦草×普通小麦F_1幼穗愈伤组织诱导和继代培养的影响[J]. 四川农业大学学报, 1992, 0(4) |
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| 作者姓名： | 伍碧华 |
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| 作者单位： | 四川农业大学小麦研究所 都江堰市611830 |
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| 摘 要： | 中间偃麦草×普通小麦F_1幼穗,在0—4℃冰箱中分别预处理5、10、15天后接种培养,结果是各个低温预处理的幼穗开始出现愈伤组织的时间都较对照提早;幼穗愈伤组织诱导率以预处理10天达最高水平。在愈伤组织继代培养到第三代时,预处理15天的老化愈伤组织频率极显著地低于对照。
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| 关 键 词： | 小麦(49EJ 61DA) 花药培养(49DB) 品种资源(49DA) 愈伤组织 中间偃麦草 低温预处理 幼穗 |
THE EFFECTS OF COLD PRETREATMENT IN INITIATION AND MAINTENANCE OF YOUNG SPIKE CALLI OF (ELYTRIGIA INTERMEDIA×TRITICUM AESTIVUM)F_1 |
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| Wu Bihua. THE EFFECTS OF COLD PRETREATMENT IN INITIATION AND MAINTENANCE OF YOUNG SPIKE CALLI OF (ELYTRIGIA INTERMEDIA×TRITICUM AESTIVUM)F_1[J]. Journal of Sichuan Agricultural University, 1992, 0(4) |
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| Authors: | Wu Bihua |
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| Abstract: | The young spikes of F1 hybrid, Elytrigia intermidia×Triticum aestivum, were treated under 0—4℃ for 5, 10 and 15 days respectively, prior to being cultured on agar medium. The results showed as follows: The explants treated showed earlier occurrence of callus than the control. The 10 days treatment expressed the highest frequency of occurrence of calli in third generation of successive culturing. The frequency of degraded calli appearing in the 15 days treatment was lower than in the control. |
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| Keywords: | WHEAT ANTHER CULTURES VARIETY RESOURCE COLD PRETREATMENT ELYTRIGIA INTERMEDIA CALLUS: YOUNG SPIKE. |
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