中草药添加剂对早期断奶仔猪生长性能和肠道菌群的影响

本试验选用28日龄杜长大断奶仔猪135头,按随机区组设计分成5组;对照组饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加0.5%、1.0%、2.0%的中草药提取物,抗生素组在基础日粮中添加20mg/kg硫酸粘杆菌素和100mg/kg杆菌肽锌,研究中草药复方对早期断奶仔猪生长性能和肠道菌群的影响。结果表明:中草药组的腹泻率比对照组分别降低47.6%、51.2%和45.2%(P0.05),与抗生素组无显著差异(P0.05)。肠道菌群DGGE图谱显示,中草药组仔猪直肠粪样相似性指数大于60%,0.5%中草药组微生物多样性最高;DGGE图谱中3条特异条带的序列与GenBank登录的相关序列的相似性都大于90%。

Effects of Chinese Herb Additives on Growth Performance and Intestinal Microflora of Weaned Piglets

To study the effects of Chinese herbs as feed additives on growth performance and intestinal bacterial diversity on piglets.135 weaned piglets(Duroc × Landrace × Yorkshire) were used in a 28-day growth trial.The piglets were allocated to five dietary treatments in a randomized complete block design.The dietary treatments were control group(basal diets);treatments of Ⅰ,Ⅱ,Ⅲ,adding 0.5%,1.0% and 2.0% of herb extract into the basal diets;antibiotic group,adding 20 mg/kg Colistin Sulfate and 100 mg/kg Bacitracin Zinc into the basal diet.The results showed that:comparing to the control group,supplementation with herb additives decreased the diarrhea by 47.6%,51.2% and 45.2%(P < 0.05),and no significant difference in the antibiotic group(P > 0.05).PCR-DGGE DNA profiles of the V6-V8 region gene of 16S rDNA of microbiota in rectum indicate the degree of similarities of Chinese herb groups were exceeding 60%,the high levels of diversity were found in 0.5% herb group;These bands indicating with a-c observed in herb groups showed exceeding 90% similarity with known sequences of Genbank.