| 赤羽病琼脂免疫扩散试验诊断方法的研究 |
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| 引用本文： | 刘焕章,李树清,陈建康.赤羽病琼脂免疫扩散试验诊断方法的研究[J].中国预防兽医学报,2000,22(4):299-301. |
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| 作者姓名： | 刘焕章 李树清 陈建康 |
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| 作者单位： | [1]中国农业科学院哈尔滨兽医研究所 [2]上海出入镜检验检疫局 |
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| 摘 要： | 应用引自美国和日本的羽病毒和标准阳性血甭，制备了琼脂免疫扩散试验（ＡＧＩＤ）抗原和高免阳性血清，建立了赤羽病ＡＧＩＤ诊断方法。应用此方法对上海、杭州、广州等地的１３８３头牛进行了检疫，ＡＧＩＤ抗体阳性牛７４６头，阳笥率５４．０％，与流行情况相符。同时对从澳大利亚、美国、新西兰和加拿大等国进口的牛、羊、猪血清１６２头份进行了检疫，全部为ＡＧＩＤ抗体阴性。
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| 关 键 词： | 赤羽病毒 琼脂免疫扩散试验 赤羽病 诊断 |
| 修稿时间： | 1999-12-30 |
Development of the AGID Test for Serodiagnosis of Akabane Disease |
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| LIU Huanzhang ,LI Shuqing ,CHEN Jiankang ,LI Jian ,WU Donglai ,LU Xiaodong ,LIU Xuezhong ,HU Yongqiang.Development of the AGID Test for Serodiagnosis of Akabane Disease[J].Chinese Journal of Preventive Veterinary Medicine,2000,22(4):299-301. |
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| Authors: | LIU Huanzhang LI Shuqing CHEN Jiankang LI Jian WU Donglai LU Xiaodong LIU Xuezhong HU Yongqiang |
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| Institution: | LIU Huanzhang 1,LI Shuqing 2,CHEN Jiankang 3,LI Jian 2,WU Donglai 1,LU Xiaodong 3,LIU Xuezhong 2,HU Yongqiang 2 |
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| Abstract: | The AGID test has been developed for the detecti
on of antibody to Akabane virus in serum.1383 cattle from Shanghai,Hangzhou and Guangzhou
were detected,746 cases shown the positive of AGID antibody,the positi ve rate was 54.0%.
Simultaneously,162 case sera of cattle,sheep and pigs importe d from Australia,America,New
Zealand and Canada were detected,all of there were negative. |
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| Keywords: | Akabane virus AGID test |
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