| 几株尖孢镰刀菌的绿色荧光蛋白基因转化 |
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| 引用本文： | 黄东杰,彭 明,李春强.几株尖孢镰刀菌的绿色荧光蛋白基因转化[J].热带农业科学,2014(1):59-62. |
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| 作者姓名： | 黄东杰 彭 明 李春强 |
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| 作者单位： | [1]中国热带农业科学院科技信息研究所, 海南海口 571101 [2]中国热带农业科学院热带生物技术研究所 ,海南海口 571101 |
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| 基金项目： | 农业部公益性行业（农业）科研专项（No.200903049-2）. |
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| 摘 要： | 利用PEG介导的原生质体转化方法,将绿色荧光蛋白基因转入到6株香蕉枯萎病菌和1株棉花枯萎病菌中。结果表明:转化子连续转接5代能够稳定遗传,荧光强度良好,PCR验证gfp基因已转入到菌株中,为后续研究香蕉枯萎病菌和棉花枯萎病菌的侵染、防治等提供可视化的检测和分析手段。
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| 关 键 词： | 尖孢镰刀菌古巴专化型 尖孢镰刀菌萎蔫专化型 绿色荧光蛋白 原生质体转化 |
Transformation of GFP Gene of Several Fusarium oxysporum Strains |
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| HUANG Dongjie,PENG Ming,LI Chunqiang.Transformation of GFP Gene of Several Fusarium oxysporum Strains[J].Chinese Journal of Tropical Agriculture,2014(1):59-62. |
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| Authors: | HUANG Dongjie PENG Ming LI Chunqiang |
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| Institution: | 1 Institute of Scientific and Technical Information, CATAS, Haikou, Hainan 571101; 2 Institute of Tropical Bioscience and Biotechnology, CATAS, Haikou, Hainan 571101) |
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| Abstract: | Several Fusarium oxysporum strains （Foc1, Foc4, Foc 8359#, Foc 8361#, Foc 8363#, Foc 8368# and Fov） have been successfully transformed with the gene coding for green fluorescent protein （GFP） using protoplast transformation method mediated by PEG. Transformants can emit quite stable fluorescence under 480 nm laser after five successive subcultures. PCR analysis confirmed that the gfp gene had been transformed into F. oxysporum strains. Those GFP transformations of F. oxysporum will provide a visible material for the study of infection and control of fusarium wilt of banana and cotton. |
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| Keywords: | Fusarium oxysporum f sp Cubence Fusarium oxysporum f sp Vasinfectum green fluorescent protein protoplast transformation |
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