| PCR法进行胚胎性别鉴定的研究进展 |
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| 引用本文： | 王晗,窦忠英. PCR法进行胚胎性别鉴定的研究进展[J]. 西北农业学报, 2005, 14(2): 5-8 |
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| 作者姓名： | 王晗 窦忠英 |
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| 作者单位： | 西北农林科技大学,国家干细胞工程技术研究中心陕西分中心,陕西,杨凌,712100 |
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| 基金项目： | 国家自然科学基金(30070374)，陕西省重点攻关项目。 |
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| 摘 要： | 通过胚胎性别鉴定,可控制家畜后代性别比例,获得巨大经济效益。早期胚胎性别鉴定的方法有,核型分析法,H-Y抗原法,X-连接酶检测法和PCR扩增法等。PCR法因其简单、快速、准确等优点,成为目前比较理想的胚胎性别鉴定方法。笔者对性别决定基因、PCR法胚胎性别鉴定的几个关键环节进行了综述,并对其前景进行了展望。
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| 关 键 词： | 胚胎 性别鉴定 活检 PCR |
| 文章编号： | 1004-1389(2005)02-0005-04 |
| 收稿时间： | 2004-10-18 |
| 修稿时间： | 2004-10-18 |
Sex Identification in Embryos with Polymerase Chain Reaction |
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| WANG Han and DOU Zhong-ying. Sex Identification in Embryos with Polymerase Chain Reaction[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2005, 14(2): 5-8 |
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| Authors: | WANG Han and DOU Zhong-ying |
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| Affiliation: | WANG Han and DOU Zhong-ying~* |
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| Abstract: | Sex identification in embryos is to achieve desired offspring sex ratio and obtain economic profits. There are several methods for sexing embryos, such as karyotype analysis, H-Y antigen detection, X-linked enzymatic determination and polymerase chain reaction (PCR) amplification. PCR-based embryo sexing are the ideal method in the present because it is relatively simple, rapid, and accurate. Sex determining gene, the key steps of sex identification in embryos with polymerase chain reaction and the perspectives were introduced in this article. |
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| Keywords: | Embryos Sex identification Biopsy PCR |
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