| PRV、PPV双重PCR检测方法的建立及其应用 |
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| 引用本文： | 曲光刚,沈志强,管宇,王金良,唐娜,谢金文.PRV、PPV双重PCR检测方法的建立及其应用[J].中国动物检疫,2007,24(11):22-23. |
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| 作者姓名： | 曲光刚 沈志强 管宇 王金良 唐娜 谢金文 |
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| 作者单位： | 1. 山东省滨州畜牧兽医研究院,山东,滨州,256600
2. 山东省滨州畜牧兽医研究院,山东,滨州,256600;山东绿都生物技术有限公司,山东,滨州,256600 |
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| 摘 要： | 为建立一种能够快速诊断猪伪狂犬病毒(PRV)、猪细小病毒(PPV)的方法,根据GenBank上发表的PRV、PPV核苷酸序列分别设计并合成了两对能特异性扩增PRV、PPV的引物,经过条件优化后,建立检测PRV、PPV的双重PCR方法,扩增两种病毒的片段分别为570bp、748bp。试验证明,该方法具有良好的特异性和敏感性,省时、省力、快速、敏感、高效的特点,为PRV与PPV的快速诊断试剂盒组装及流行病学的研究提供了技术支持。
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| 关 键 词： | 双重PCR 猪伪狂犬病毒 猪细小病毒 |
| 文章编号： | 1005-944X（2007）11-0022-02 |
The Establishment and Application of a Double PCR for PRV and PPV |
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| QU Guanggang,SHEN Zhiqiang,GUAN Yu,WANG Jinliang,TANG n,XIE jinwen.The Establishment and Application of a Double PCR for PRV and PPV[J].China Journal Of Animal Quarantine,2007,24(11):22-23. |
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| Authors: | QU Guanggang SHEN Zhiqiang GUAN Yu WANG Jinliang TANG n XIE jinwen |
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| Abstract: | According to the nucleotide sequences pseudorabies virus (PRV) and porcine parvoviruses (PPV) in Gen-Bank,two pairs of primers were designed and synthesized.A double PCR was developed based on the two primer pairs of primers which amplified the PRV virus-specific segment with the size 570bp and the PPV virus-specific segment with the size 748bp after the PCR conditions were optimized. The results indicated that the double PCR show a good foundation of sensitive and special assay for the clinical diagnosis and epidemiological research. |
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| Keywords: | double PCR pseudorabies virus porcine parvoviruses |
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