| 日本对虾杆状核病毒性传染病诊断技术 |
| |
| 引用本文： | Kazuo Momoyama,李常勇. 日本对虾杆状核病毒性传染病诊断技术[J]. 河北渔业, 1997, 0(2): 5-10 |
| |
| 作者姓名： | Kazuo Momoyama 李常勇 |
| |
| 作者单位： | Kazuo Momoyama，Midori Hiraoka，Kiyoshi Inouye，Takeshi Kimura and Heiji NakanoYamaguchi Prefectural Naikai Fisheries Experiment Station，Yainaguchi 754，Japan National Research Institute of Aquaculture，Inland Station，Taniaki，Mie 519-04，JapanKumamoto Prefectural Fisheries Research Center，Senouchi，Ohyano-cho，Amakusa-gun，Kumamoto 869-36，Japan |
| |
| 摘 要： | 本文提出了分别用暗视野显微技术和透射电子显微技术快速确诊日本对虾杆状核病毒性传染病的方法。在暗视野显微镜下观察到的血淋巴中的病毒粒子大小约为0.5μm;胃表层上皮被病毒感染的细胞核明显地呈白色均质小体,直径10～15μm,球形或椭圆形,坏死细胞被囊化为棕色小块(约20～50μm)。病虾血淋巴和胃经负染制样在透射电子显微镜下观察病毒颗粒大小为400×150nm;核衣壳大小为390×85nm。
|
| 关 键 词： | 诊断技术 杆状核病毒 日本对虾 |
Diagnostic Techniques of the Rod-shaped Nuclear Virus Infection in the Kuruma Shrimp, Penaeus japonicus |
| |
| Kazuo Momoyama,Midori Hiraoka,Kiyoshi Inouye,Takeshi Kimura and Heiji NakanoYamaguchi Prefectural Naikai Fisheries Experiment Station,Yainaguchi ,Japan National Research Institute of Aquaculture,Inland Station,Taniaki,Mie -,JapanKumamoto Prefectural Fisheries Research Center,Senouchi,Ohyano-cho,Amakusa-gun,Kumamoto -,Japan. Diagnostic Techniques of the Rod-shaped Nuclear Virus Infection in the Kuruma Shrimp, Penaeus japonicus[J]. Hebei Fisheries, 1997, 0(2): 5-10 |
| |
| Authors: | Kazuo Momoyama Midori Hiraoka Kiyoshi Inouye Takeshi Kimura Heiji NakanoYamaguchi Prefectural Naikai Fisheries Experiment Station Yainaguchi Japan National Research Institute of Aquaculture Inland Station Taniaki Mie - JapanKumamoto Prefectural Fisheries Research Center Senouchi Ohyano-cho Amakusa-gun Kumamoto - Japan |
| |
| Affiliation: | Kazuo Momoyama,Midori Hiraoka,Kiyoshi Inouye,Takeshi Kimura and Heiji NakanoYamaguchi Prefectural Naikai Fisheries Experiment Station,Yainaguchi 754,Japan National Research Institute of Aquaculture,Inland Station,Taniaki,Mie 519-04,JapanKumamoto Prefectural Fisheries Research Center,Senouchi,Ohyano-cho,Amakusa-gun,Kumamoto 869-36,Japan |
| |
| Abstract: | Rapid and confirmative diagnostic methods for the rod-shaped nuclear virus (RV-PJ) infection in the kuruma shrimp (Penaeus juponicus) by dark-field microscopy and transmission electron microscopy, respectively, were proposed.Under a dark-field microscope virus particles were seen as numerous fine particles (about 0. 5/μm) in the hemolymph. In stomach cutichlar epidermis, virus-infected nuclei were seen as sharply outlined homogenous white bodies,10-15μm in diameter, round or oblong in shape and hemocytic encapsulations of necrotic cells as small brown masses (about 20-50μm). Under a transmission electron microscope virus particles were seen as virions (400×150mm) or capsids (390×85nm) in negatively stained preparations from hemolymph and stomach. |
| |
| Keywords: | diagnostic techniques RV-PJ virus kuruma shrimp Penaeus japonicus |
| 本文献已被 CNKI 等数据库收录！ |
|
