| 西农莎能奶山羊和布尔山羊GnRH基因多态性与产羔数的相关分析 |
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| 引用本文： | 颜泉梅,陈秋菊,崔易虹,朱广琴,朱春梅,安小鹏,杨莎,王建刚,宋宇轩,曹斌云. 西农莎能奶山羊和布尔山羊GnRH基因多态性与产羔数的相关分析[J]. 中国兽医学报, 2011, 31(11) |
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| 作者姓名： | 颜泉梅 陈秋菊 崔易虹 朱广琴 朱春梅 安小鹏 杨莎 王建刚 宋宇轩 曹斌云 |
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| 作者单位： | 西北农林科技大学动物科技学院,陕西杨凌,712100 |
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| 基金项目： | 国家高技术研究发展计划(863)项目(2007AA10Z167) |
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| 摘 要： | 根据牛GnRH基N序列设计4对引物，采用PCR—SSCP技术检测GnRH基因的多态性，同时用最小二乘法研究了其多态性与产羔数和生长体质量的关系。所研究的西农莎能奶山羊和布尔山羊群体在P4扩增片段存在多态性，分别定义为CC、CT和TT3种基因型；P1、P2和P3扩增片段均不存在多态性，只表现一种带型。通过测序发现CC型与GG型相比扩增片段的第185、336bp处同时有C—T的单碱基突变。在西农莎能奶山羊和布尔山羊群体中，CC和CT基因型产羔数最小二乘均值均极显著或显著高于TT基因型（P〈0．05）。研究结果表明，GnRH基因对西农莎能奶山羊和布尔山羊的产羔数均有显著影响，因此认为GnRH基因P4扩增位点的突变可作为西农莎能奶山羊和布尔山羊多胎性状的有效分子标记。
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| 关 键 词： | GnRH PCR—SSCP 遗传多态性 西农萨能奶山羊 布尔山羊 |
Polymorphism of GnRH gene and its relationship with little size and growth weight of xinong shaaneng dairy goat and boer goat |
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| YAN Quan-mei,CHEN Qiu-ju,CUI Yi-hong,ZHU Guang-qin,ZHU Chun-mei,AN Xiao-peng,YANG Sha,WANG Jian-gang,SONG Yu-xuan,CAO Bin-yun. Polymorphism of GnRH gene and its relationship with little size and growth weight of xinong shaaneng dairy goat and boer goat[J]. Chinese Journal of Veterinary Science, 2011, 31(11) |
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| Authors: | YAN Quan-mei CHEN Qiu-ju CUI Yi-hong ZHU Guang-qin ZHU Chun-mei AN Xiao-peng YANG Sha WANG Jian-gang SONG Yu-xuan CAO Bin-yun |
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| Affiliation: | YAN Quan-mei,CHEN Qiu-ju,CUI Yi-hong,ZHU Guang-qin,ZHU Chun-mei,AN Xiao-peng,YANG Sha,WANG Jian-gang,SONG Yu-xuan,CAO Bin-yun(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | 4 pairs primers were designed to detect single nucleotide polymorphism of GnRH gene in both xinong shaaneng dairy goat breed and boer goat breed by PCR-SSCP and,sequencing was applied to analyze the genetic variation,and meanwhile least square was applied to analyze the relation between different genotypes and the little size and growth weight.The results showed that only the product amplified by primer 4(P4) displayed polymorphism,and three genotypes(CC,CT and TT) were detected in both two goat breeds.For ... |
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| Keywords: | GnRH PCR-SSCP genetic polymorphism xinong shaaneng dairy goat boer goat |
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