| 鸡新城疫重组鸡痘病毒活疫苗的免疫持续期和加强免疫研究 |
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| 引用本文： | 甘军纪,陶鸽,张胜文,吴永启,彭大新,刘秀梵.鸡新城疫重组鸡痘病毒活疫苗的免疫持续期和加强免疫研究[J].中国兽医学报,2011,31(12). |
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| 作者姓名： | 甘军纪 陶鸽 张胜文 吴永启 彭大新 刘秀梵 |
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| 作者单位： | 扬州大学兽医学院,江苏扬州,225009 |
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| 基金项目： | 国家863计划(2006AA10A205); 江苏省高技术研究计划(BG2007336) |
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| 摘 要： | 本研究旨在评价表达新城疫病毒（NDV）血凝素-神经氨酸酶（HN）基因的重组鸡痘病毒（rFPV-12LSHN）活疫苗的免疫持续期和加强免疫对疫苗免疫效力的影响。用rFPV-12LSHN活疫苗免疫14日龄SPF鸡,103PFU/羽,7d即可检测到NDV HI抗体应答,对NDV强毒F48E8株攻毒保护率达100%。一次免疫18周后,对NDV强毒攻击依然提供完全保护。鸡痘病毒（FPV）疫苗免疫4周,再接种rFPV-12LSHN活疫苗,攻毒保护率降低至50%。相反,rFPV-12LSHN免疫4周,随后二次免疫可显著提高对NDV的体液免疫应答水平（P〈0.01）,对NDV强毒攻击的保护率仍然为100%。结果表明,表达NDV HN基因的重组鸡痘病毒（rFPV-12LSHN）活疫苗,能够快速建立坚强免疫力,免疫持续期至少可达18周,rFPV-12LSHN的二次免疫可以提高疫苗的免疫力。
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| 关 键 词： | 新城疫病毒 重组鸡痘病毒 疫苗 持续期 加强免疫 |
Duration of immunity and boost vaccination of a recombinant fowlpox virus expressing Newcastle disease virus HN gene |
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| GAN Jun-ji,TAO Ge,ZHANG Sheng-wen,WU Yong-qi,PENG Da-xin,LIU Xiu-fan.Duration of immunity and boost vaccination of a recombinant fowlpox virus expressing Newcastle disease virus HN gene[J].Chinese Journal of Veterinary Science,2011,31(12). |
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| Authors: | GAN Jun-ji TAO Ge ZHANG Sheng-wen WU Yong-qi PENG Da-xin LIU Xiu-fan |
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| Institution: | GAN Jun-ji,TAO Ge,ZHANG Sheng-wen,WU Yong-qi,PENG Da-xin,LIU Xiu-fan(College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China) |
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| Abstract: | To evaluate the duration of immunity and the efficacy of boost vaccination of a recombinant fowlpox virus(rFPV-12LSHN) expressing HN gene from a Newcastle disease virus(NDV) isolate(ZJ1).Two-week old SPF chickens vaccinated with one dose of vaccine containing 103 plaque forming units(PFU) of rFPV-12LSHN were completely protected from virulent NDV after 7 days vaccination,hemagglutination inhibition(HI) antibody against NDV could be detected as early as 7 days postvaccination and immune protection lasted up ... |
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| Keywords: | Newcastle disease virus recombinant fowlpox virus vaccine duration boost vaccination |
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