| 猪三毛滴虫长春株不同染色方法的比较 |
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| 引用本文： | 李伟志,李文超,王泽东,宫鹏涛,李建华,张西臣. 猪三毛滴虫长春株不同染色方法的比较[J]. 中国兽医学报, 2011, 31(9) |
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| 作者姓名： | 李伟志 李文超 王泽东 宫鹏涛 李建华 张西臣 |
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| 作者单位： | 吉林大学畜牧兽医学院,吉林长春,130062 |
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| 基金项目： | 国家自然科学基金资助项目(30970322) |
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| 摘 要： | 将从长春地区腹泻仔猪盲肠中分离得到的猪三毛滴虫用1640培养基短期培养后,使用碘染色法、瑞氏染色法、姬氏染色法、姬氏-瑞氏复染法、Weigere铁苏木素染色法和鞭毛染色法染色分别对其染色,观察不同虫体形态结构,并比较了不同染色方法的染色效果。结果显示,姬氏染色、瑞氏-姬氏复染和鞭毛染色法对猪三毛滴虫染色效果较好,可获得虫体形态完整、结构清晰的染色标本。
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| 关 键 词： | 猪三毛滴虫 染色 比较 |
A comparison between different staining methods of Trichomonad suis Changchun isolate |
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| LI Wei-zhi,LI Wen-chao,,WANG Ze-dong,GONG Peng-tao,LI Jian-hua,ZHANG Xi-chen. A comparison between different staining methods of Trichomonad suis Changchun isolate[J]. Chinese Journal of Veterinary Science, 2011, 31(9) |
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| Authors: | LI Wei-zhi LI Wen-chao WANG Ze-dong GONG Peng-tao LI Jian-hua ZHANG Xi-chen |
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| Affiliation: | LI Wei-zhi1,LI Wen-chao1,2,WANG Ze-dong1,GONG Peng-tao1,LI Jian-hua1,ZHANG Xi-chen1 (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.College of Animal Science,Anhui Science and Technology University,Fengyang,Anhui 233100,China) |
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| Abstract: | An isolate of Trichomonad suis was isolated from diarrhea pig colon in Changchun.After the isolate was cultured for a short-term,Tritrichomonas suis changchun isolate was stained by six staining methods including the iodine staining,Wright's stain,Giemsa stain,Giemsa-Wright staining,Weigert's iron hematoxylin staining and flagella staining method.we observed the morphological structure of the isolate and compared the effects of different staining methods.The results showed that Giemsa staining,Wright-Giemsa... |
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| Keywords: | Tritrichomonas suis stain comparison |
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