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| 牛蛙（Rana catesbeiana）皮肤抗菌肽的cDNA克隆及序列分析 | |
| 引用本文： | 赵瑞利,韩俊友,韩文瑜,雷连成,江丽娜,张俊敏,欧阳萍. 牛蛙（Rana catesbeiana）皮肤抗菌肽的cDNA克隆及序列分析[J]. 中国兽医学报, 2011, 31(10) |
| 作者姓名： | 赵瑞利 韩俊友 韩文瑜 雷连成 江丽娜 张俊敏 欧阳萍 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,吉林长春130062／天津农学院动物科学系,天津300384 2. 吉林大学植物科学学院,吉林长春,130062 3. 吉林大学畜牧兽医学院,吉林长春,130062 |
| 基金项目： | 国家自然科学基金资助项目(30571416) |
| 摘 要： | 为了解牛蛙皮肤抗菌肽的多样性及结构特点,根据GenBank数据库中蛙属抗菌肽基因信号肽序列设计简并引物,以RT-PCR技术扩增牛蛙皮肤抗菌肽基因,克隆得到cDNA全长序列并进行测序和序列分析。用生物信息学软件分析其cDNA序列特点,预测成熟肽的理化性质。结果得到48个完整的cDNA序列,分别编码24种抗菌肽前体,它们均由信号肽、前导肽和成熟肽3部分组成。对序列测定结果的预测分析表明,牛蛙皮肤抗菌肽前体核苷酸序列与已报道的其他蛙类抗菌肽具有较高的同源性,同源性达到80%以上,但成熟肽的序列变异很大,具有物种特异性,其中6个为新的抗菌肽序列。牛蛙皮肤抗菌肽成熟肽的二级结构主要以α-螺旋和β-折叠为主,均为疏水性短肽。 |
| 关 键 词： | 牛蛙 抗菌肽 cDNA克隆 序列分析 |
cDNA clone and sequence analysis of antibacterial peptides in skin of Rana catesbeiana |
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| ZHAO Rui-li,,HAN Jun-you,HAN Wen-yu,LEI Lian-cheng,JIANG Li-na,ZHANG Jun-min,OUYANG Ping. cDNA clone and sequence analysis of antibacterial peptides in skin of Rana catesbeiana[J]. Chinese Journal of Veterinary Science, 2011, 31(10) | |
| Authors: | ZHAO Rui-li HAN Jun-you HAN Wen-yu LEI Lian-cheng JIANG Li-na ZHANG Jun-min OUYANG Ping |
| Affiliation: | ZHAO Rui-li1,2,HAN Jun-you3,HAN Wen-yu1*,LEI Lian-cheng1,JIANG Li-na1,ZHANG Jun-min1,OUYANG Ping1(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.Department of Animal Science,Tianjin Agricultural University,Tianjin 300384,3.College of Plant Science,China) |
| Abstract: | To analyze the diversity and construction features of the skin antibacterial peptides of Rana catesbeiana,the degenerate primers were designed based on the signal peptide of Rana antibacterial peptides and subjected to RT-PCR amplification of corresponding cDNAs from Rana catesbeiana mRNA.cDNAs were cloned and sequenced.Properties of matured peptides were predicted by using informatics software tools.48 complete cDNAs were found to encode 19 antimicrobial peptides precursor including the signal peptide,prod... |
| Keywords: | Rana catesbeiana antibacterial peptide cDNA cloning sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |