| 鸡咽部混合菌群16S rDNA序列分析方法的建立 |
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| 引用本文： | 蔡亚娜,刘玉庆,胡明,魏甜甜,白华,高超.鸡咽部混合菌群16S rDNA序列分析方法的建立[J].中国兽医学报,2011,31(9). |
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| 作者姓名： | 蔡亚娜 刘玉庆 胡明 魏甜甜 白华 高超 |
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| 作者单位： | 山东省农业科学院畜牧兽医研究所山东省动物疫病防治与繁育重点试验室,山东济南,250100 |
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| 基金项目： | 国家自然科学基金资助项目(30770044); 山东省优秀中青年科学家科研奖励基金资助项目(2006BCO2008) |
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| 摘 要： | 通过优化鸡咽拭子预处理方法、储存条件、提取咽拭子标本中的细菌总DNA条件、细菌16SrDNA保守区通用引物设计及PCR扩增条件,成功建立了鸡咽部菌群16SrDNA序列分析方法,并初步分析了健康鸡的咽部菌群种类和组成,同时发现鸡咽部菌群中未知细菌和未可培养细菌所占的比例很大,需要深入研究其对鸡呼吸道疾病的影响。
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| 关 键 词： | 鸡咽部标本 混合菌群 16S rDNA 序列分析 未可培养细菌 |
Development of 16S rDNA sequence analysis method for mixed bacterial flora in chicken throat samples |
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| CAI Ya-na,LIU Yu-qing,HU Ming,WEI Tian-tian,BAI Hua,GAO Chao.Development of 16S rDNA sequence analysis method for mixed bacterial flora in chicken throat samples[J].Chinese Journal of Veterinary Science,2011,31(9). |
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| Authors: | CAI Ya-na LIU Yu-qing HU Ming WEI Tian-tian BAI Hua GAO Chao |
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| Institution: | CAI Ya-na1,2,LIU Yu-qing1,HU Ming2,WEI Tian-tian1,BAI Hua2,GAO Chao1,2 (1.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao,Shandong 266109,China,2.Shandong Provincial Key Laboratory of Animal Disease Control & Breeding,Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Science,Jinan 250100,China) |
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| Abstract: | Based on optimization design of pretreatment conditions of chicken swabs for the total bacteria DNA extracted effectively,primers amplified for 16S rDNA and its PCR procedure,16S rDNA sequence analysis was developed and applied to analyze mixed bacterial flora in chicken throat samples.It was demonstrated that there were lots of viable but nonculturable or even no-known species in throat bacteria,which might be involved in mixed respiractory tract infections for chicken. |
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| Keywords: | chicken throat swab mixed bacterial flora 16S rDNA sequence analysis nonculturable bacteria |
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