| 马立克氏病病毒对鸡肿瘤相关基因TNFSF13B和ST18表达水平的影响 |
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| 引用本文： | 孙红艳,连玲,陈燕梅,刘长军,张莹,杨宁,曲鲁江.马立克氏病病毒对鸡肿瘤相关基因TNFSF13B和ST18表达水平的影响[J].中国兽医学报,2011,31(10). |
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| 作者姓名： | 孙红艳 连玲 陈燕梅 刘长军 张莹 杨宁 曲鲁江 |
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| 作者单位： | 1. 中国农业大学动物科技学院,北京,100193
2. 中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001 |
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| 基金项目： | “十二五”国家科技支撑计划资助项目(2011BAD28B03); 现代农业产业技术体系建设专项资金资助项目(CARS-41); 家禽产业技术体系北京市创新团队专项资金资助项目 |
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| 摘 要： | 将150只1日龄雏鸡随机分成2组,100只作为攻毒组,50只作为对照组。攻毒组腹腔注射2 000PFU的马立克氏病病毒（MDV）-GA细胞毒0.2mL;对照组注射同等剂量的病毒稀释液。2组鸡严格隔离,在相同条件的负压隔离器中饲养。攻毒后60d,屠宰所有存活鸡。在对照组中取4个正常鸡的脾脏（HS）;在攻毒组中取4个感染MDV后产生肿瘤鸡的脾脏（TS）及4个感染MDV后未产生肿瘤并表现为健康鸡的脾脏（ANS）,并对TNFSF13B和ST18基因在这些脾脏组织中的表达水平进行荧光定量分析。结果表明,肿瘤相关基因TNFSF13B和ST18的表达在产生肿瘤鸡的脾脏中都是显著性下调的。MDV通过抑制宿主TNFSF13B基因的表达来抑制B细胞分化、成熟进而对宿主免疫系统进行抑制;同时MDV通过下调肿瘤抑制基因ST18的表达,促使机体肿瘤发生。在对MDV有明显抵抗能力的鸡（ANS组）中,TNFSF13B和ST18的表达水平与对照组健康鸡基本一致,表明这2个基因在MDV抗病过程中起着重要作用。
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| 关 键 词： | 鸡 MDV-GA TNFSF13B ST18 |
Differential expression of tumor-related genes TNFSF13B and ST18 in chickens infected with Marek's disease virus |
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| SUN Hong-yan,LIAN Ling,CHEN Yan-mei,LIU Chang-jun,ZHANG Ying,YANG Ning,QU Lu-jiang.Differential expression of tumor-related genes TNFSF13B and ST18 in chickens infected with Marek's disease virus[J].Chinese Journal of Veterinary Science,2011,31(10). |
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| Authors: | SUN Hong-yan LIAN Ling CHEN Yan-mei LIU Chang-jun ZHANG Ying YANG Ning QU Lu-jiang |
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| Institution: | SUN Hong-yan1,LIAN Ling1,CHEN Yan-mei1,LIU Chang-jun2,ZHANG Ying1,YANG Ning1,QU Lu-jiang1*(1.College of Animal Science and Technology,China Agricultural University,Beijing 100193,China,2.Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | One hundred and fifty 1-day-old chicks were divided into two groups randomly.Exprimental group(100 chicks) were infected intraperitoneally with 2 000 plaque-forming units(PFU) of the MDV-GA.The uninfected control group(50 chicks) were injected with the same dosage of diluent(0.2 mL).The two groups were housed in separate cages in the same filtered-air,positive-pressure isolation rooms.At the end of trial period(60 d.p.i.),we dissected all the survivals,and chose healthy spleens(HS) from four age-matched uni... |
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| Keywords: | chicken MDV-GA TNFSF13B ST18 |
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