| 湖南猪源粪肠球菌的分离鉴定及16S rDNA系统进化分析 |
| |
| 引用本文： | 黎满香,林荣高,薛立群,蒋伟,陈滔.湖南猪源粪肠球菌的分离鉴定及16S rDNA系统进化分析[J].中国兽医学报,2011,31(9). |
| |
| 作者姓名： | 黎满香 林荣高 薛立群 蒋伟 陈滔 |
| |
| 作者单位： | 湖南农业大学动物医学院,湖南长沙,410128 |
| |
| 基金项目： | 湖南省科技计划资助项目(2010NK3015) |
| |
| 摘 要： | 从湖南各地送检至实验室的临床样本中分离到42株革兰氏染色阳性及过氧化氢酶阴性的球菌,其中78.6%来自于肺脏,菌落形态及染色镜检均与粪肠球菌参考株ATCC 29212相似,兰氏分群鉴定97.6%（41/42）为D群,生化特性符合粪肠球菌特征。16SrDNA测序鉴定显示：42个分离株与同步测序的ATCC 29212同源性在99.2%到100%之间,与所选择粪肠球菌参考序列的同源性在98.7%到100%之间,NCBI在线BLAST分析发现42株均与GeneBank收录的粪肠球菌序列同源性最高。湖南分离株16SrDNA序列与E.faecalis参考序列进化关系与地域分布无关,来源于不同宿主的菌株的16SrDNA变异并不大,而与E.faecium、E.canis、E.avium、E.hirae等4种肠球菌则有多处变异,这些变异区域在这4种肠球菌中却是保守的。
|
| 关 键 词： | 粪肠球菌 鉴定 16S rDNA |
Finding and 16S rDNA phyletic evolution analysis of Enterococcus faecalis isolated from swine in Hunan province |
| |
| LI Man-xiang,LIN Rong-gao,XUE Li-qun,JIANG Wei,CHEN Tao.Finding and 16S rDNA phyletic evolution analysis of Enterococcus faecalis isolated from swine in Hunan province[J].Chinese Journal of Veterinary Science,2011,31(9). |
| |
| Authors: | LI Man-xiang LIN Rong-gao XUE Li-qun JIANG Wei CHEN Tao |
| |
| Institution: | LI Man-xiang,LIN Rong-gao,XUE Li-qun,JIANG Wei,CHEN Tao (College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China) |
| |
| Abstract: | In this study,Gram's staining of positive and catalase negative coccus strains(n=42) was isolated from clinical diseased pig in Hunan province,most of those strains were from lung(78.6%).The colonial morphology and the shape of Cram strain bear a close analogy to ATCC29212 strain.Lancefield test showed that 97.6%(41/42)strains belongs to group D.The sequence analysis of 16S rDNA revealed that all Hunan strains share a high identity with reference strain ATCC29212(99.2%)and the homology ranged from 98.7% to ... |
| |
| Keywords: | Enterococcus faecalis identification 16S rDNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
