海南龙血树的组织培养与快速繁殖

以海南龙血树的顶芽和侧芽作为外植体，把其接种于MS＋BA1mg/L＋NAA0．1mgg/L＋PVP100 mg/L＋蔗糖30g／L培养基上培养40-50d可诱导其腋芽萌发，再把萌发后所形成的新芽切割下来接种于MS＋BA 2mg／L＋KT 0．5mg／L＋蔗糖30g／L的培养基上培养25-30d可诱导形成丛生芽，丛生芽在继代培养过程中每25-30d可增殖3～5倍。把丛生芽分割成单株并接种于MS＋BA3mg厂L＋GA，1mg／L＋蔗糖30g／L培养基上培养4周后使其壮苗后再接种于MS＋NAA0．5～1．0mg／L＋蔗糖30g／L培养基上培养4周可诱导小芽形成完整的根系，小植株移栽成活率可达98％。该体系的建立为海南龙血树的工厂化育苗奠定了坚实的基础。

Tissue Culture and Rapid Propagation of Dracaena cambodiana Pierre ex Gagnep.

The apical buds and axillary buds of Dracaena cambodiana Pierre ex Gagnep. were inoculated on MS medium containing 1 mg/L BA, 0.1 mg/L NAA, 100 mg/L PVP and 30 g/L sucrose for inducing shoots. After cultured for 40-50 d, the shoots bourgeoned. They were then transferred onto MS medium supplemented with 2 mg/L BA, 0.5 mg/L KT and 30 g/L sucrose for inducing clustered buds. After 25-30 d, the clustered buds formed. The proliferation rate of the clustered buds was 300 %-500 % per month. The clustered buds were cultured on MS medium containing 3 mg/L BA, 1 mg/L GA3 and 30 g/L sucrose for about 30 d to make the shoots stronger. These shoots were transferred onto MS medium containing 0.5-1.0 mg/L NAA and 30 g/L sucrose for 25-30 d for inducing roots. About 98 % of plantlets survived after they were transferred onto soil. This cultural system will be a good foundation for commercial proliferation of D. cambodiana.