| 农杆菌介导法将LFY基因导入苹果的研究 |
| |
| 引用本文： | 刘静,邵建柱,徐继忠,李湘利,郭文武. 农杆菌介导法将LFY基因导入苹果的研究[J]. 河北农业大学学报, 2006, 29(3): 6-9 |
| |
| 作者姓名： | 刘静 邵建柱 徐继忠 李湘利 郭文武 |
| |
| 作者单位： | 河北农业大学,园艺学院,河北,保定,071001;济宁师范专科学校,生物系,山东,济宁,272025;华中农业大学,作物遗传改良国家重点实验室,湖北,武汉,430070 |
| |
| 基金项目： | 河北省科技厅科技攻关项目 , 河北农业大学校科研和教改项目 |
| |
| 摘 要： | 以M26苹果叶片为外植体,利用GUS瞬时表达率,对农杆菌介导法转化苹果的因素进行优化,通过含LFY基因的根癌农杆菌EHA105转化苹果叶片,获得了提早开花的转基因植株。结果表明:以OD600=0.3~0.5农杆菌菌液侵染叶片,25℃条件下共培养,共培养后的叶片延迟筛选3 d,在附加卡那霉素30 mg/L的再生培养基中选择培养,转化芽再生率可达6.97%。PCR检测初步证明目的LFY基因已整合到苹果基因组中。
|
| 关 键 词： | 苹果 农杆菌 遗传转化 LFY GUS |
| 文章编号： | 1000-1573（2006）03-0006-04 |
| 收稿时间： | 2005-12-12 |
| 修稿时间： | 2005-12-12 |
Study on the transformation of apple with LFY gene mediated by Agrobacterium tumefaciens |
| |
| LIU Jing,SHAO Jian-zhu,XU Ji-zhong,LI Xiang-li,GUO Wen-wu. Study on the transformation of apple with LFY gene mediated by Agrobacterium tumefaciens[J]. Journal of Agricultural University of Hebei, 2006, 29(3): 6-9 |
| |
| Authors: | LIU Jing SHAO Jian-zhu XU Ji-zhong LI Xiang-li GUO Wen-wu |
| |
| Abstract: | Apple rootstock of M_(26) was transformed by Agrobacterium tumefaciens EHA105 carrying LFY gene.The factors affecting apple transformation were studied through GUS transient expression,such as different co-cultivation temperature,different bacterium density,et al.Leaf pieces were infected with Agrobacterium OD_(600)=0.3～0.5,then cocultivated at 25℃.After 3 d delayed selection the leaf pieces were incubated on regeneration medium containing 30 mg/L kanamycin.The regeneration rate of transformation buds reached 6.97%.It was proved by PCR analysis that the target LFY gene had been integrated into the genome of regenerated plants. |
| |
| Keywords: | apple Agrobacterium tumefaciens genetic transformation LFY GUS |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
