| 玉米CMS–C型雄性不育系C543恢复基因的初定位 |
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| 引用本文： | 刘伟华,罗红兵,邱博.玉米CMS–C型雄性不育系C543恢复基因的初定位[J].湖南农业大学学报(自然科学版),2016,42(1):16-19. |
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| 作者姓名： | 刘伟华 罗红兵 邱博 |
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| 作者单位： | 1. 湖南农业大学农学院,湖南长沙,410128;2. 湖南农业大学农学院,湖南长沙410128;湖南农业大学南方粮油作物协同创新中心,湖南长沙410128 |
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| 基金项目： | "十二·五"农村领域国家科技计划项目，湖南省科技计划项目，长沙市科技计划项目 |
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| 摘 要： | 以玉米恢复系614、B137分别与CMS–C型雄性不育系C543组配的F2代(614群体和B317群体)为试材,进行花粉育性鉴定和遗传分析。花粉育性镜检发现,C543花药内没有花粉,属于无花粉型雄性不育;遗传分析表明,C543的不育性状由1对隐性核基因控制,恢复系614和B137各有1对核恢复基因;SSR标记连锁分析结果将恢复基因定位在玉米8号染色体短臂上,位于标记umc1483附近,两者距离约16.9 c M。
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| 关 键 词： | 玉米 雄性不育系 质核互作 连锁分析 基因定位 |
Primary mapping of restoring gene for CMS-C cytoplasmic male sterility line C543 in maize |
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| Abstract: | Two F2 generations, which from the hybridizations of C543×614 and C543×B137, were used as experiment materials to analyze the pollen fertility and inheritance. The results showed that C543 was pollen-free male sterility, which was controlled by a pair of recessive nuclear genes, and there was a restoring gene in restorer 614 and B137, respectively. With the analyzing of SSR, the restoring gene of B137 was mapped on short arm of chromosome 8 and 16.9 cM from the marker umc1483. |
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| Keywords: | maize male sterility line cytoplasm-nuclear interaction linkage analysis gene mapping |
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