| 24份水稻细胞质雄性不育系的SSR多态性分析 |
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| 引用本文： | 郭慧,李树杏,徐建第,张红宇,徐培洲,吴先军.24份水稻细胞质雄性不育系的SSR多态性分析[J].杂交水稻,2007,22(3):56-61. |
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| 作者姓名： | 郭慧 李树杏 徐建第 张红宇 徐培洲 吴先军 |
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| 作者单位： | 四川农业大学,水稻研究所,四川,温江,611130;四川农业大学,西南作物基因资源与遗传改良教育部重点实验室,四川,雅安,625014 |
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| 基金项目： | 教育部长江学者和创新团队发展计划 |
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| 摘 要： | 利用SSR标记技术对四川省及其它部分省区生产中主要使用的和新育成的24份水稻细胞质雄性不育系材料进行遗传多样性分析,从135对SSR引物中筛选出具有多态性的引物34对,共扩增出77个等位位点,平均每个引物的等位位点数为2.26个;多态信息含量（PIC）变化范围0.080～0.559,平均为0.254。根据24份细胞质雄性不育系的遗传相似系数矩阵做出树状图,聚类分析结果表明,目前在生产中应用的水稻细胞质雄性不育系遗传背景比较单一,不利于充分发挥水稻杂种优势利用潜力。
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| 关 键 词： | 杂交水稻 细胞质雄性不育系 SSR标记 遗传多样性 聚类分析 |
| 文章编号： | 1005-3956（2007）03-0056-06 |
| 修稿时间： | 2006-10-20 |
SSR Marker-based Genetic Diversity of 24 Cytoplasmic Male Sterile Lines in Rice |
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| GUO Hui,LI Shu-xing,XU Jian-di,ZHANG Hong-yu,XU Pei-zhou,WU Xian-jun.SSR Marker-based Genetic Diversity of 24 Cytoplasmic Male Sterile Lines in Rice[J].Hybrid Rice,2007,22(3):56-61. |
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| Authors: | GUO Hui LI Shu-xing XU Jian-di ZHANG Hong-yu XU Pei-zhou WU Xian-jun |
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| Abstract: | The SSR analysis was conducted on the genetic diversity of 24 major cytoplasmic male sterile(CMS) lines of hybrid rice that were newly developed or widely used in production in Sichuan and some other provinces.Out of 135 random primers used in this study,34 displayed well in polymorphism.On the 34 SSR loci,a total of 77 allelic loci were detected.Every primer pairs offered 2.26 allelic loci on average.The value of allelic polymorphism information content(PIC) ranged from 0.08 to 0.559,on an average of 0.254.The cluster analysis was conducted and a dendrogram of the tested 24 CMS lines was constructed by UPMGA based on the genetic similarity coefficients determined by SSR analysis.The results showed that the genetic diversity was relatively poor among the existing CMS lines,which is not favorable to make full use of rice heterosis. |
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| Keywords: | hybrid rice CMS lines SSR markers genetic diversity cluster analysis |
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