| ISSR分子标记技术在植物病原菌研究中的应用 |
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| 引用本文： | 文静. ISSR分子标记技术在植物病原菌研究中的应用[J]. 安徽农业科学, 2010, 38(36): 20658-20660. DOI: 10.3969/j.issn.0517-6611.2010.36.060 |
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| 作者姓名： | 文静 |
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| 作者单位： | 赤峰学院生命科学系,内蒙古赤峰,024000 |
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| 基金项目： | 赤峰学院引进人才项目基金 |
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| 摘 要： | ISSR分子标记是PCR基础上发展起来的一种新技术,其基本原理是在SSR的5′或3′端加锚1～4个嘌呤或嘧啶碱基,然后以此为引物对两侧具有反向排列的一段基因组序列进行扩增。阐述了ISSR的原理和特点,并论述了ISSR标记技术目前在植物病原菌分类、亲缘关系和遗传多样性等方面的研究及应用。
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| 关 键 词： | 分子标记 ISSR 植物病原菌 |
Application of ISSR Molecular Marker Technique in Plant Pathogens Research |
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| WEN Jing. Application of ISSR Molecular Marker Technique in Plant Pathogens Research[J]. Journal of Anhui Agricultural Sciences, 2010, 38(36): 20658-20660. DOI: 10.3969/j.issn.0517-6611.2010.36.060 |
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| Authors: | WEN Jing |
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| Affiliation: | WEN Jing (Department of Life Science; Chifeng University; Chifeng; Inner Mognia 024000); |
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| Abstract: | Inter-simple sequence repeat(ISSR)marker is a new molecular marker based on Polymerase Chain Reaction(PCR).The technique involves amplification of genomic segments flanked by inversely oriented and closely spaced micro-satellite sequences by a single primer or a pair of primers based on SSR′ s anchored 5′ or 3′ with 1-4 purine or pyrimidine bases.The principles and characteristics of ISSR were described,and its application in plant pathogens classification of system,genetic relationship and genetic diversity were discussed. |
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| Keywords: | ISSR |
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