| 诺卡氏菌产高活力腈水合酶补料分批发酵工艺的优化 |
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| 引用本文： | 黄伟波,黎常宏,王筱兰.诺卡氏菌产高活力腈水合酶补料分批发酵工艺的优化[J].安徽农业科学,2010,38(36):20562-20565. |
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| 作者姓名： | 黄伟波 黎常宏 王筱兰 |
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| 作者单位： | 江西师范大学生命科学学院,江西南昌,330022;昌九农科化工有限公司,江西南昌,330022 |
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| 摘 要： | 目的]优化诺卡氏菌产腈水合酶的发酵工艺,获得高活力的腈水合酶。方法]对诺卡氏菌(Nocardiasp.)发酵产腈水合酶过程中菌体生长、pH变化以及葡萄糖消耗对腈水合酶合成的影响进行了分析。结果]在分批补糖工艺中,使发酵液的葡萄糖浓度维持在1.5～6 g/L,发酵结束时,发酵液酶活达到2 814.3 U/ml,是优化前的8.7倍。结论]分批补糖工艺增加了菌生长的生物量,大大提高了诺卡氏菌的产酶水平。
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| 关 键 词： | 腈水合酶 分批补糖工艺 诺卡氏菌 |
The Optimization of Fed-batch Fermentation for Production of High Activity Nitrite Hydratase by Nocardia sp. |
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| Institution: | HUANG Wei-bo et al(College of Life Science; Jiangxi Normal University; Nanchang; Jiangxi 330022); |
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| Abstract: | Objective]The aim was to optimize the technology of producing the nitrile hydratase by Nocardia sp.and achieve the high activity nitrile hydratase.Method] The effects of the cell growth,pH and glucose consumption on fermenting of Nocardia sp.were studied.Result]The results showed that maintain the concentation of glucose 1.5-6 g/L for the glucose fed-batch fermentation,the nitrile hydratase activity was 2 814.3 U/ml which was 8.7 times before optimization.Conclusion]The novel technology could increase the biomass of bacteria growth and greatly improved the enzyme production levels of Nocardia sp.For the glucose fed-batch fermentation |
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| Keywords: | Nitrile hydratase Glucose fed-batch Nocardia sp |
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