中草药复合添加剂对羊瘤胃消化酶活性的影响

为了研究自制中草药复合添加剂对绵羊瘤胃消化酶活性的影响,将60只4月龄健康杜寒杂一代绵羊随机分为4组进行试验,分别为公羊、母羊对照组(常规日粮),公羊、母羊试验组(常规日粮+中草药复合添加剂),试验期为45d。试验结束后取绵羊瘤胃液,分别测定瘤胃蛋白酶、羧甲基纤维素酶、α-淀粉酶活性。结果表明,该中草药复合添加剂对绵羊瘤胃蛋白酶、α-淀粉酶活性的影响均不显著(P>0.05),但显著(P<0.05)提高了绵羊瘤胃羧甲基纤维素酶的活性,且上述3种酶活性的变化均与绵羊性别无关。研究结果提示,该中草药复合添加剂能提高绵羊瘤胃羧甲基纤维素酶的活性,可促进饲草料中纤维素在羊瘤胃内的消化代谢。

Effect of Dietary Supplementation of a Chinese Herbal Medicine Compound Additive on Activity of Ruminal Digestive Enzymes in Sheep

In an attempt to investigate the impact of dietary supplementation of a Chinese herbal medicine compound additive on activity of ruminal digestive enzymes in sheep,a total of 60 four-month-old healthy Dorper and Small-tailed Han sheep crossbred lambs (F1) of were randomly assigned into the following four groups: the male and female control groups were fed with the basal diet,and the male and female experimental groups fed the basal diet supplemented with Chinese herbal medicine compound additive.The feeding experiment lasted for 45 days.At the end of the experiment,the rumen fluid samples were collected from the lambs,and the activity of protease,carboxymethyl cellulase and alpha-amylase in rumen fluid of the lambs in different groups were determined and statistically analyzed.The results demonstrated that while the dietary supplementation of Chinese herbal medicine compound additive had no significant impact on activity of ruminal protease and alpha-amylase in the crossbred lambs (P>0.05),it significantly elevated the activity of ruminal carboxymethyl cellulase (P<0.05).In addition,the variation of the three tested ruminal digestive enzymes expressed no association with the lambs′ gender.Our results suggest that the dietary supplementation of Chinese herbal medicine compound additive is able to improve the activity of ruminal carboxymethyl cellulase in lambs and thus can promote the digestion and metabolism of forage cellulose in sheep rumen.