HPLC法测定植物源杀螨浴剂(外用)中氧化苦参碱的含量

目的]建立HPLC法测定植物源杀螨浴剂(外用)中的氧化苦参碱含量。方法]采用Waters XBridge C18(4.6mm×150mm,5μm)色谱柱,流动相为甲醇-0.05mol/L磷酸二氢钠(含0.5%高氯酸钠)(15∶85),流速为1.0mL/min,检测波长为220nm,进样量为10μL,柱温为30℃。结果]氧化苦参碱质量浓度在0.020592~0.45747mg/mL(Y=5.72e+006X+1.13e+004,R^2=0.9998)与峰面积积分值呈良好的线性关系;精密度试验RSD为0.3%(n=6);重复性试验RSD为1.0%(n=6);回收率试验RSD为1.6%。结论]该法测定氧化苦参碱含量简便、快捷,稳定性好,重复性高,可用于控制植物源杀螨浴剂(外用)的质量。

Determination of Oxymatrine Content in a Botanical Acaricidal Bath Agent for External Use by HPLC

Objective] To establish a HPLC method for determination of oxymatrine content in a botanical acaricidal bath agent for external use.Method] The separation was performed with the commercially available chromatographic column of Waters XBridge C18 (4.6 mm×150 mm,5 μm).Methanol-0.05 mol/L sodium dihydrogen phosphate (containing 0.5% sodium perchlorate)(15:85) was used as mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was 220 nm,the sample volume was 10 μL,and the column temperature was 30 ℃.Result]There was a good linearity between the peak areas and the mass concentrations of oxymatrine in the range of 0.020 592 - 0.457 47 mg/mL (Y=5.72e+006X+1.13e+004,R^2=0.9998).The RSD of the precision test (n=6),repeatability test (n=6) and recovery test was 0.3%,1.0% and 1.6%,respectively.Conclusion] The established HPLC method has the advantages of simplicity,speed,good stability and high repeatability,and can be used in quality control of the botanical acaricidal bath agent for external use.