| 基于双平台的InDel标记玉米杂交种纯度鉴定方法 |
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| 引用本文： | 梁紫越,刘志浩,马世鹏,赵怡锟,许理文,康定明,王凤格.基于双平台的InDel标记玉米杂交种纯度鉴定方法[J].玉米科学,2022,30(3):32-39. |
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| 作者姓名： | 梁紫越 刘志浩 马世鹏 赵怡锟 许理文 康定明 王凤格 |
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| 作者单位： | 中国农业大学农学院, 北京 100193;北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097;北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京 100097;黑龙江大学现代农业与生态环境学院, 哈尔滨 150080 |
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| 基金项目： | 北京市农林科学院科技创新能力建设专项（KJCX20200305） |
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| 摘 要： | 种子纯度是种子质量检测重要指标，也是玉米生产中利用玉米杂种优势，获得高产稳产的重要保证。开发建立一套高效、高通量和低成本的玉米杂交种纯度鉴定方法，是我国玉米生产应用中迫切需要解决的问题。选用郑单958、京科968、农大108等10个玉米杂交品种为材料，利用20个InDel分子标记，建立了一套玉米杂交种纯度筛检分离的鉴定方法。该方法利用荧光毛细管电泳平台组建的多重PCR及多重电泳，结合KASP平台鉴定玉米杂交种纯度，实现高效、高通量和低成本的目标，经验证KASP平台纯度鉴定结果与预期结果一致。
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| 关 键 词： | 玉米 杂交种 InDel标记 纯度鉴定 荧光毛细管电泳 KASP |
| 收稿时间： | 2021/4/19 0:00:00 |
Using Indel Markers for Purity Identification Method of Hybrid Seeds in Maize Based on Dual Platforms |
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| LIANG Zi-yue,LIU Zhi-hao,MA Shi-peng,ZHAO Yi-kun,XU Li-wen,KANG Ding-ming,WANG Feng-ge.Using Indel Markers for Purity Identification Method of Hybrid Seeds in Maize Based on Dual Platforms[J].Journal of Maize Sciences,2022,30(3):32-39. |
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| Authors: | LIANG Zi-yue LIU Zhi-hao MA Shi-peng ZHAO Yi-kun XU Li-wen KANG Ding-ming WANG Feng-ge |
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| Institution: | College of Agriculture, China Agricultural University, Beijing 100193;Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097;Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097;School of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China |
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| Abstract: | Seed purity is the most important of the four major seed quality inspection indicators, but also a guarantee for utilizing maize heterosis in maize production to obtain high and stable yields. To this end, there is an urgent need to develop a high-efficient, high-throughput, and low-cost method for maize hybrid purity identification in our country''s maize production. In this study, 10 maize hybrid varieties, including Zhengdan958, Jingke968, and Nongda108, were used as materials, and using 20 InDel molecular markers to develop a method of the screening and identification separated for maize hybrids purity identification. This method uses multiplex PCR and multiple fluorescent capillary electrophoresis and the KASP platform to identify the purity in maize hybrids, which is high-efficient, high-throughput, and low-cost. The identification results are consistent with the expectation. |
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| Keywords: | Maize Hybrid InDel marker Purity identification Fluorescent capillary electrophoresis KASP |
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