| 甘薯根腐病抗病性室内鉴定方法的研究 |
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| 引用本文： | 谢逸萍.甘薯根腐病抗病性室内鉴定方法的研究[J].植物保护,1999(6). |
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| 作者姓名： | 谢逸萍 |
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| 作者单位： | 江苏徐州甘薯研究中心!221121 |
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| 基金项目： | 国家“九五”科技攻关项目 |
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| 摘 要： | 将PDA 平面培养的甘薯根腐病菌研磨成浆,按一定比例加入蛭石中,接菌最适浓度为每200 g蛭石加1/ 2 培养皿菌浆,随后栽入薯苗,在25 ℃下培养25 ～30 d 后检查。以薯苗叶片枯黄程度及根部黑根死根多少划分病级,以病级平均数确定品种抗性,同时对抗感品种接种根腐病菌后过氧化物酶活性进行测定。试验证明,上述方法可以简便、快速、准确地测定品种对根腐病的抗性,同时也证明过氧化物酶活性作为量化分级标准的可行性。
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| 关 键 词： | 甘薯根腐病 抗病性 过氧化物酶 |
Studies on the inoculation methods for testing the resistance of sweetpotato varieties to root rot in laboratory. |
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| Xie Yiping.Studies on the inoculation methods for testing the resistance of sweetpotato varieties to root rot in laboratory.[J].Plant Protection,1999(6). |
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| Authors: | Xie Yiping |
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| Abstract: | The pathogen solution from PDA slate medium mixed with sweetpotato cuttings was transplanted into vermiculits. Observation was done 25-30 days after the transplanting. The yellowish and blackness leaves of roots were scaled. The activity of peroxide was tested on inoculated plants. The results showed that big difference existed between varieties with resistance or susceptibility, and the activity of susceptible varieties was found to be higher than that of varieties with high resistance. Peroxide activity could be used as an indirect index to evaluate the root rot resistance. |
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| Keywords: | sweet potato root rot resistance inoculation method |
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