利用荧光探针和激光共聚焦显微镜显示玉米根冠细胞微丝分布与变化

利用罗丹明标记的鬼笔环肽为荧光探针,通过激光共聚焦荧光显微镜,观察了玉米根冠细胞中微丝的分布与变化。结果显示:脱落的玉米根冠细胞中存在微丝网络,纤细的微丝相互交织,并与荧光颗粒交叉相连,其中在细胞核、质体等细胞器周围较密集,可见放射状微丝束,暗示微丝的功能与细胞核、细胞器的活动相关。细胞松弛素B可破坏微丝分布;来自玉米小斑病菌C小种的致病毒素对微丝网络也有专化破坏作用,并且它们对微丝的破坏作用随着浓度的增大而加强。

Distribution and Variation of Microfilaments in the Root Cap Cells of Maize under CLSM

The distribution and variation of microfilaments(MFs) in the maize root cap detached cells were examined under confocal laser scanning microscopy(CLSM) by using TRITC-phalloidin(TRITC-Ph) as fluorescent probe.The results showed that the microfilaments network was distributed on the root cells.The microfilaments were interweaved each other and connected with fluorescent particles.The microfilaments networks were dense and some radiative MFs bundles distributed around the nucleus and organelles.This suggested that the microfilaments were correlative with their function.Both CB and HMC toxin,a host-specific toxin from Bipolaris(Helminthosporium) maydis race C,could destroy the MFs distribution and had obvious quantity-effects relations.