| 将新的人工雄性不育基因导入小麦栽培品种的研究初报 |
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| 引用本文： | 李艳红,肖兴国,赵广荣,聂秀玲,张爱民,阎隆飞. 将新的人工雄性不育基因导入小麦栽培品种的研究初报[J]. 农业生物技术学报, 1999, 7(3): 255-258 |
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| 作者姓名： | 李艳红 肖兴国 赵广荣 聂秀玲 张爱民 阎隆飞 |
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| 作者单位： | 1. 植物生理生化开放实验室
2. 植物遗传育种系,中国农业大学,北京,100094 |
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| 摘 要： | 利用ＰＤＳ－１０００型氢气基因枪将我们构建的雄性不育基因导入小麦栽培品种的幼胚愈伤组织，经含除草剂Ｂａｓｔａ的抗性培养基分化和生根筛选后得到２７株绿苗，ＰＣＲ和Ｓｏｕｒｈｔｅｍ检测均发现有３种株呈阳性，初步表明基因已整合到小麦的基因组中，转化频率达０．２％左右。
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| 关 键 词： | 基因枪 雄性不育基因 小麦 |
Preliminary Study of Transferring New Engineered Male Sterile Gene into Cultivated Wheat |
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| Li Yanhong,Xiao Xingguo,Zhao Guangrong,Nie Xiuling,Zhang Aimin,Yan Longfei. Preliminary Study of Transferring New Engineered Male Sterile Gene into Cultivated Wheat[J]. Journal of Agricultural Biotechnology, 1999, 7(3): 255-258 |
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| Authors: | Li Yanhong Xiao Xingguo Zhao Guangrong Nie Xiuling Zhang Aimin Yan Longfei |
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| Abstract: | A newly engineered male sterile genewas transferred into immature embryo - derived calli of commercial wheat cultivars by PDS-1000/He gene-gun. Twenty-seven green plants were recovered and rooted on Basta containingmedium in one and half month. More than 95 % of regenerated plants survived aftertransplanting in pots and three of them were PCR and Southern positive, showingintegration of the male sterile gene into wheat genome at a rate of about 0.2 %. |
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