| 当归RAPD反应条件的优化 |
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| 引用本文： | 盛丽,王蒂,司怀军.当归RAPD反应条件的优化[J].甘肃农业大学学报,2005,40(5):591-595. |
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| 作者姓名： | 盛丽 王蒂 司怀军 |
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| 作者单位： | 1. 甘肃农业大学农学院,甘肃,兰州,730070;甘肃省作物遗传改良与种质创新重点实验室,甘肃,兰州,730070
2. 甘肃省作物遗传改良与种质创新重点实验室,甘肃,兰州,730070;甘肃农业大学生命科学技术学院,甘肃,兰州,730070 |
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| 摘 要： | 以当归DNA为模板,对影响当归RAPD扩增的重要参数进行了优化试验,以期建立当归RAPD反应的最适体系。通过正交法及单因素试验对RAPD反应条件进行优化,7个试验因子的最适条件为:模板DNA 0.5 ng/μL,引物0.8μmol/L,dNTPs 0.25 mmol/L,MgCl22.5 mmol/L,Taq酶0.8 U,退火温度36~37℃,循环次数40。
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| 关 键 词： | 当归 基因组DNA RAPD |
| 文章编号： | 1003-4315(2005)05-0591-05 |
| 修稿时间： | 2005年3月3日 |
Optimization of RAPD reaction conditions for Angelica sinensis |
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| SHENG Li,WANG Di,SI Huai-jun.Optimization of RAPD reaction conditions for Angelica sinensis[J].Journal of Gansu Agricultural University,2005,40(5):591-595. |
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| Authors: | SHENG Li WANG Di SI Huai-jun |
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| Abstract: | Regard DNA of Angelica sinensis as the template,several important parameters influencing RAPD of A.sinensis were studied in order to set up the optimum RAPD system of A.sinensis.Seven essential factors that affected the results of RAPD were optimized by orthogonal experiment and signal factor experiment.The results showed that the optimalum conditions 0.5 ng/μL DNA,0.8 μmol/L primer,0.25 mmol/L dNTPs,2.5 mmol/L MgCl_(2),0.8 U Taq polymerase,36～37 ℃ annealing temperature and 40 cycles. |
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| Keywords: | Angelica sinensis genomic DNA RAPD |
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