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| 烟草种间原生质体融合及其影响因素的研究 | |
| 引用本文： | 陈名红,陈毅坚,陈学军,吴渝生.烟草种间原生质体融合及其影响因素的研究[J].热带农业科技,2007,30(2):29-32. |
| 作者姓名： | 陈名红 陈毅坚 陈学军 吴渝生 |
| 作者单位： | 1. 云南民族大学,化学与生物技术学院,昆明,650031 2. 云南烟草科学研究院,昆明,650106 3. 云南农业大,农学与生物技术学院,昆明,650201 |
| 摘 要： | 利用PEG-高Ca2+高pH法对烟草属的普通烟草K326和Nicotiana rustica、N.alata、N.plumbaginifolia、N.debneyi分别进行种间叶肉原生质体融合试验,结果表明基因型对烟草叶肉原生质体融合有明显影响。通过试验还对影响烟草叶肉原生质体融合及其成株的其它相关因素进行了探讨。 |
| 关 键 词： | 烟草 原生质体融合 影响因素 |
| 文章编号： | 1672-450X（2007）02-0029-04 |
| 修稿时间： | 2007-04-22 |
Study on Interspecific Protoplast Fusion and Its Factors of Tabacco |
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| CHEN Ming-hong,CHEN Yi-jian,CHEN Xue-lun,WU Yu-sheng.Study on Interspecific Protoplast Fusion and Its Factors of Tabacco[J].Tropical Agricultural Science & Technology,2007,30(2):29-32. | |
| Authors: | CHEN Ming-hong CHEN Yi-jian CHEN Xue-lun WU Yu-sheng |
| Institution: | 1. The School of Chemistry and Biotechnology of Yunnan, Kunming 650031, China; 2. Yunnan Academy of Tabacco Science, Kunming 650106, China; 3. The School of Agronomy and Biotechnology of YAU, Kunming 650201, China |
| Abstract: | In this paper tobacco interspecific protoplast fusion was studied with mesophyll protoplasts isolated from K326 and N.rustica,N.alata,N.plumbaginifolia,N.debneyi respectively by PEG-high Ca2 /pH.The obtained results showed that genotype affected tobacco protoplast fusion obviously.Some other factors affecting protoplast fusion and plant regeneration were also studied here. |
| Keywords: | tabacco protoplast fusion plant regeneration influencing factor |
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