| 转化CaMV基因芸薹属蔬菜植株抗病性鉴定及其遗传分析 |
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| 引用本文： | 巩振辉,宋献军,张广辉,张桂华,何玉科.转化CaMV基因芸薹属蔬菜植株抗病性鉴定及其遗传分析[J].园艺学报,2001,28(5):466-468. |
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| 作者姓名： | 巩振辉 宋献军 张广辉 张桂华 何玉科 |
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| 作者单位： | ( 西北农林科技大学园艺学院, 杨凌712100; 􀀁 中国科学院上海植物生理研究所, 上海200032) |
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| 基金项目： | 国家自然科学基金资助项目(39770522);教育部资助优秀年轻教师基金项目 |
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| 摘 要： | 以转化CaMV 弱株系Bari1 基因􀀂 的大白菜、菜薹、紫菜薹和花椰菜植株为试材, 研究了CaMV 弱株系Bari􀀁1 基因􀀂 所提供的遗传工程交叉保护及其遗传规律。结果表明,在转化CaMV 弱株系Bari􀀁1 基因􀀂 的大白菜、菜薹、紫菜薹和花椰菜植株中, 48. 08%对CaMV强株系CABB􀀁BJI 具有较强的抗性, 51. 92%表现为敏感。抗性基因( CaMV Bari􀀁1 基因) 在自交代( S1) 中大多数( 76%) 表现为典型的孟德尔单基因显性遗传, 部分株系的抗性出现了15 1, 1 1, 1 3 和1 95 的非孟德尔遗传现象。
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| 关 键 词： | CaMV 基因 芸薹属 转基因 植株 遗传工程交叉保护 遗传规律 |
| 文章编号： | 0513-353(2001)05-0466-03 |
| 修稿时间： | 2001年2月5日 |
Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus in Brassica Crops Which Was Transformed with CaMV Gene VI |
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| Gong Zhenhui ,He Yuke ,Song Xianjun ,Zhang Guanghui ,and Zhang Guihua.Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus in Brassica Crops Which Was Transformed with CaMV Gene VI[J].Acta Horticulturae Sinica,2001,28(5):466-468. |
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| Authors: | Gong Zhenhui He Yuke Song Xianjun Zhang Guanghui and Zhang Guihua |
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| Institution: | (Horticulture College, Northwest Sci􀀁Tech University of Agriculture and Forestry , Yangling 712100; Shanghai Institute of Plant Physiology , The Chinese Academy of Science, Shanghai 200032) |
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| Abstract: | The genetically engineered cross􀀁protection which was supplied by weak strain Bari􀀁1 gene VI of cauliflower mosaic virus ( CaMV) , and its genetic regularity were studied with Brassica crops ( Brassica camp estris ssp.rap ifera, B . campestris ssp. pekinensis , B . campestris var . parachinensis , B. campestris var. purpurea, B .juncea , B . oleracea var. botrytic ) which were transformed with CaMV gene VI. The results showed that 48. 08% oftransformed Brassica plants was resistance to CaMV strain CABB􀀁BJI and 51. 92% of them was susceptible to CaMV CABB􀀁BJI. The virus resistant gene ( CaMV Bari􀀁1 gene VI ) in 76% of first selfed progeny presented typicallyMendelian single dominance gene inheritance. The ratio of virus􀀁resistant to virus􀀁susceptible plants in some of first selfed progeny presented Mendelian inheritance, such as, 15 1, 1 1, 1 3 and 1 95. |
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| Keywords: | CaMV gene VI Brassica L Transgenic Plants Genetically engineered cross protection Genetic regularity |
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