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转化CaMV基因芸薹属蔬菜植株抗病性鉴定及其遗传分析
引用本文:巩振辉,宋献军,张广辉,张桂华,何玉科. 转化CaMV基因芸薹属蔬菜植株抗病性鉴定及其遗传分析[J]. 园艺学报, 2001, 28(5): 466-468
作者姓名:巩振辉  宋献军  张广辉  张桂华  何玉科
作者单位:( 西北农林科技大学园艺学院, 杨凌712100; 􀀁 中国科学院上海植物生理研究所, 上海200032)
基金项目:国家自然科学基金资助项目(39770522);教育部资助优秀年轻教师基金项目
摘    要: 以转化CaMV 弱株系Bari1 基因􀀂 的大白菜、菜薹、紫菜薹和花椰菜植株为试材, 研究了CaMV 弱株系Bari􀀁1 基因􀀂 所提供的遗传工程交叉保护及其遗传规律。结果表明,在转化CaMV 弱株系Bari􀀁1 基因􀀂 的大白菜、菜薹、紫菜薹和花椰菜植株中, 48. 08%对CaMV强株系CABB􀀁BJI 具有较强的抗性, 51. 92%表现为敏感。抗性基因( CaMV Bari􀀁1 基因) 在自交代( S1) 中大多数( 76%) 表现为典型的孟德尔单基因显性遗传, 部分株系的抗性出现了15 1, 1 1, 1 3 和1 95 的非孟德尔遗传现象。

关 键 词:CaMV 基因  芸薹属  转基因  植株  遗传工程交叉保护  遗传规律
文章编号:0513-353(2001)05-0466-03
修稿时间:2001-02-05

Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus in Brassica Crops Which Was Transformed with CaMV Gene VI
Gong Zhenhui ,He Yuke ,Song Xianjun ,Zhang Guanghui ,and Zhang Guihua. Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus in Brassica Crops Which Was Transformed with CaMV Gene VI[J]. Acta Horticulturae Sinica, 2001, 28(5): 466-468
Authors:Gong Zhenhui   He Yuke   Song Xianjun   Zhang Guanghui   and Zhang Guihua
Affiliation:(Horticulture College, Northwest Sci􀀁Tech University of Agriculture and Forestry , Yangling 712100; Shanghai Institute of Plant Physiology , The Chinese Academy of Science, Shanghai 200032)
Abstract:The genetically engineered cross􀀁protection which was supplied by weak strain Bari􀀁1 gene VI of cauliflower mosaic virus ( CaMV) , and its genetic regularity were studied with Brassica crops ( Brassica camp estris ssp.rap ifera, B . campestris ssp. pekinensis , B . campestris var . parachinensis , B. campestris var. purpurea, B .juncea , B . oleracea var. botrytic ) which were transformed with CaMV gene VI. The results showed that 48. 08% oftransformed Brassica plants was resistance to CaMV strain CABB􀀁BJI and 51. 92% of them was susceptible to CaMV CABB􀀁BJI. The virus resistant gene ( CaMV Bari􀀁1 gene VI ) in 76% of first selfed progeny presented typicallyMendelian single dominance gene inheritance. The ratio of virus􀀁resistant to virus􀀁susceptible plants in some of first selfed progeny presented Mendelian inheritance, such as, 15 1, 1 1, 1 3 and 1 95.
Keywords:CaMV gene VI  Brassica L.  Transgenic  Plants  Genetically engineered cross protection  Genetic regularity
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