Simple tube centrifugation for processing platelet-rich plasma in the horse |
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| Authors: | Robin L Fontenot Carolyn A Sink Stephen R Werre Nicole M Weinstein Linda A Dahlgren |
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| Institution: | Department of Large Animal Clinical Sciences (Fontenot, Dahlgren), Veterinary Teaching Hospital (Sink), Laboratory for Study Design and Statistical Analysis (Werre), and Department of Biomedical Sciences and Pathobiology (Weinstein), Phase II Duck Pond Drive, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061-0442, USA. |
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| Abstract: | This study evaluated the quality and bacteriologic safety of platelet-rich plasma (PRP) produced by 3 simple, inexpensive tube centrifugation methods and a commercial system. Citrated equine blood collected from 26 normal horses was processed by 4 methods: blood collection tubes centrifuged at 1200 and 2000 × g, 50-mL conical tube, and a commercial system. White blood cell (WBC), red blood cell (RBC), and platelet counts and mean platelet volume (MPV) were determined for whole blood and PRP, and aerobic and anaerobic cultures were performed. Mean platelet concentrations ranged from 1.55- to 2.58-fold. The conical method yielded the most samples with platelet concentrations greater than 2.5-fold and within the clinically acceptable range of > 250 000 platelets/λL. White blood cell counts were lowest with the commercial system and unacceptably high with the blood collection tubes. The conical tube method may offer an economically feasible and comparatively safe alternative to commercial PRP production systems. |
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