| 犬源贾第虫EF1α基因的克隆及序列分析 |
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| 引用本文： | 李结,王培园,张萍,刘远佳,郭建超,孟祥龙,李国清.犬源贾第虫EF1α基因的克隆及序列分析[J].动物医学进展,2012(1):53-56. |
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| 作者姓名： | 李结 王培园 张萍 刘远佳 郭建超 孟祥龙 李国清 |
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| 作者单位： | 华南农业大学兽医学院 |
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| 基金项目： | 国家自然科学基金项目(30972179) |
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| 摘 要： | 以广州某宠物医院临床犬源贾第虫为研究对象,根据GenBank TM中登录的贾第虫EF1α基因序列(AF069575),设计1对引物EF/ER,采用PCR方法从贾第虫基因组DNA中扩增出目的片段,纯化后克隆至pGEM-T Easy载体,重组质粒通过菌液PCR鉴定后,对其进行序列测定及分析,旨在对广州犬源贾第虫进行分子鉴定。结果显示,该犬源贾第虫EF1α序列长为288bp,与预期目的片段一致,该序列构建的分子进化树表明该犬源贾第虫分离株为蓝氏贾第虫D型。说明EF1α适合做分子标记,可准确地对贾第虫进行基因分型,为贾第虫分子分类学及分子遗传学研究奠定了基础。
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| 关 键 词： | 犬源贾第虫 EF1α基因 克隆 PCR技术 序列分析 |
Cloning and Sequence Analysis of EF1α Gene of Giardia lamblia from Dogs |
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| LI Jie,WANG Pei-yuan,ZHANG Ping,LIU Yuan-jia,GUO Jian-chao,MENG Xiang-long,LI Guo-qing.Cloning and Sequence Analysis of EF1α Gene of Giardia lamblia from Dogs[J].Progress In Veterinary Medicine,2012(1):53-56. |
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| Authors: | LI Jie WANG Pei-yuan ZHANG Ping LIU Yuan-jia GUO Jian-chao MENG Xiang-long LI Guo-qing |
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| Institution: | (College of Veterinary Medicine,South China Agricultural University,Guangzhou,Guangdong,510642,China) |
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| Abstract: | In order to amplify the EF1α gene of Giardia lamblia isolated from dogs in Guangzhou,one pair of primers EF/ER were designed based on the published sequence of the EF1α gene in GenBankTM and the amplicons were cloned into pGEM-T easy vector.The recombinant plasmids were identified by PCR and the products were sequenced and analyzed.The results show that the EF1α of the dog-derived Giardia strain is 288 bp in length.The phylogenetic tree revealed that the dog-derived Giardia strain was Giardia lamblia Assemblage D.These results provided a foundation for further studies of molecular identification and molecular genetics of Giardia lamblia. |
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| Keywords: | Giardia lamblia EF1α cloning PCR amplification sequence analysis |
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