Effect of allelic variation at the Glu-3/Gli-1 loci on breadmaking quality parameters in hexaploid wheat (Triticum aestivum L.) |
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| Affiliation: | 1. Instituto de Recursos Biológicos, CIRN, Instituto Nacional de Tecnología Agropecuaria (INTA), De Los Reseros y N. Repetto, 1686 Hurlingham, Buenos Aires, Argentina;2. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600 Mexico, D.F., Mexico;3. Department of Plant Sciences, University of California, Davis, CA 95616-8780, USA;4. Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA;1. Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, PR China;2. Bruker (Beijing) Scientific Technology Co., Ltd., Beijing 100081, PR China;1. United States Department of Agriculture (USDA), Agricultural Research Service (ARS)-CSWQRU, Soft Wheat Quality Laboratory, 1680 Madison Avenue, Wooster, OH, 44691, USA;2. Department of Horticulture and Crop Science, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA;1. Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Minhang District, Shanghai, 201106, PR China;2. Plant Biology and Crop Science Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom;3. Computational and Systems Biology Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom;1. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA;2. Nutrition & Food Science Department, Texas A&M University, College Station, TX 77843, USA;3. USDA-ARS Center for Grain and Animal Health Research, Manhattan, KS 66502, USA |
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| Abstract: | Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at the Gli-1 locus. Using DNA markers and a backcross program, we developed a set of nine near isogenic lines (NILs) including different Glu-A3/Gli-A1 or Glu-B3/Gli-B1 alleles in the genetic background of the Argentine variety ProINTA Imperial. The nine NILs and the control were evaluated in three different field trials in Argentina. Significant genotype-by-environment interactions were detected for most quality parameters indicating that the effects of the Glu-3/Gli-1 alleles are modulated by environmental differences. None of the NILs showed differences in total flour protein content, but relative changes in the abundance of particular classes of proteins cannot be ruled out. On average, the Glu-A3f, Glu-B3b, Glu-B3g and Glu-B3iMan alleles were associated with the highest values in gluten strength-related parameters, while Glu-A3e, Glu-B3a and Glu-B3iChu were consistently associated with weak gluten and low quality values. The value of different Glu-3/Gli-1 allele combinations to improve breadmaking quality is discussed. |
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| Keywords: | Low molecular weight glutenins Gliadins Wheat Breadmaking quality A-PAGE" },{" #name" :" keyword" ," $" :{" id" :" kwrd0035" }," $$" :[{" #name" :" text" ," _" :" acid-polyacrylamide gel electrophoresis BC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" backcross FPC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" flour protein content GPC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" grain protein content HMW-GS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" high-molecular-weight glutenin subunits LMW-GS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" low-molecular-weight glutenin subunits MDS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" midline descending slope MPA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" midline peak area MPH" },{" #name" :" keyword" ," $" :{" id" :" kwrd0115" }," $$" :[{" #name" :" text" ," _" :" midline peak height MPT" },{" #name" :" keyword" ," $" :{" id" :" kwrd0125" }," $$" :[{" #name" :" text" ," _" :" midline peak time NILs" },{" #name" :" keyword" ," $" :{" id" :" kwrd0135" }," $$" :[{" #name" :" text" ," _" :" near isogenic lines PCR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0145" }," $$" :[{" #name" :" text" ," _" :" polymerase chain reaction SDS-PAGE" },{" #name" :" keyword" ," $" :{" id" :" kwrd0155" }," $$" :[{" #name" :" text" ," _" :" sodium dodecyl sulfate polyacrylamide gel electrophoresis STS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0165" }," $$" :[{" #name" :" text" ," _" :" sequence tagged site TKW" },{" #name" :" keyword" ," $" :{" id" :" kwrd0175" }," $$" :[{" #name" :" text" ," _" :" thousand-kernel weight ZEL" },{" #name" :" keyword" ," $" :{" id" :" kwrd0185" }," $$" :[{" #name" :" text" ," _" :" micro Zeleny sedimentation test |
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