英国薰衣草愈伤再生体系的建立

目的]建立英国薰衣草愈伤再生体系,为薰衣草规模化快繁和开展转基因工作奠定基础.方法]以英国薰衣草(Lavandula angusti folia Mill.) 为材料,建立了以叶片为外植体的愈伤再生体系.结果] 以MS +2,4-D 0.10 mg/L + KT 0.30 mg/L为培养基诱导愈伤组织效果好; 把经过2～3次继代的淡黄色愈伤组织在MS+6-BA 2.0 mg/L+NAA 0.50 mg/L培养基上继续培养,30 d后形成大量的丛生芽.待小芽长至1～2 cm后放入MS+6-BA 1.0 mg/L扩繁培养基中扩繁1～2代. 扩繁后的小芽放入1/2 MS+IAA 1.0 mg/L 培养基上进行生根培养,20 d后生根率达 93.33;.结论]得到完整再生植株,能大量快繁英国薰衣草.

Establishment of Callus Regeneration System of English Lavender

Objective and Metod]In this experiment,the callus regeneration system of Lavender was established by the use of it as meterial,which layed the basis for fast reproduction of Lavender and genetic transformation.Result]It was shown that the induction results of the MS +2,4-D 0.10 mg/L + KT0. 30 mg/L medium groups were better than that of other groups.The yellowish callus from 2-3 generations were continuously cultivated on the medium of MS+6-BA 2.0 mg/L+NAA 0.50 mg/L,which form great number of thickly-growing root,when young bud reached 1-2 cm,then was put in medium with MS+6-BA 1.0 mg/L to be expanded for 1-2 generations.After that,the young bud was put in medium with 1/2 MS+IAA 1.0 mg/L to continue to be cultiveated to be rooting.Conclusion]In 20 days,the rooting rate reached 33%.