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Improved detection sensitivity using an optimal eDNA preservation and extraction workflow and its application to threatened sawfishes
Authors:Madalyn K. Cooper  Roger Huerlimann  Richard C. Edmunds  Alyssa M. Budd  Agnès Le Port  Peter M. Kyne  Dean R. Jerry  Colin A. Simpfendorfer
Affiliation:1. Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland, Australia;2. Centre for Tropical Water and Aquatic Ecosystem Research (TropWATER), James Cook University, Townsville, Queensland, Australia;3. Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland, Australia

Centre for Tropical Bioinformatics and Molecular Biology, James Cook University, Townsville, Queensland, Australia;4. Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland, Australia

Centre for Tropical Water and Aquatic Ecosystem Research (TropWATER), James Cook University, Townsville, Queensland, Australia;5. Research Institute for the Environment and Livelihoods, Charles Darwin University, Darwin, Northern Territory, Australia

Abstract:
    Keywords:conservation genetics  eDNA  method optimization  Pristidae  qPCR  threatened species
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