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High lysyl oxidase expression is an indicator of poor prognosis in dogs with cutaneous mast cell tumours |
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| Authors: | Julia Antongiovanni Joselevitch Thiago Henrique Moroni Vargas Lidia Hildebrand Pulz Karine Germano Cadrobbi Greice Cestari Huete Adriana Tomoko Nishiya Silvia Regina Kleeb José Guilherme Xavier Ricardo De Francisco Strefezzi |
| Institution: | 1. Laboratório de Oncologia Comparada e Translacional, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil;2. Laboratório de Oncologia Comparada e Translacional, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil
Faculdade de Medicina Veterinária e Zootecnia, Departamento de Patologia, Universidade de São Paulo, São Paulo, Brazil;3. Laboratório de Oncologia Comparada e Translacional, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, Brazil Clínica E+ Especialidades, São Paulo, Brazil;4. Universidade Anhembi Morumbi, São Paulo, Brazil;5. Universidade Anhembi Morumbi, São Paulo, Brazil Universidade Metodista de São Paulo, São Bernardo do Campo, Brazil;6. Universidade Paulista, São Paulo, Brazil |
| Abstract: | Mast cell tumour (MCT) is one of the most frequent skin tumours in dogs. Due to their unpredictable biological behaviour, MCTs often cause several therapeutic frustrations, leading to investigation regarding prognostic markers. Lysyl oxidase (LOX) is an enzyme that promotes extracellular matrix stability and contributes to cell migration, angiogenesis and epithelial-mesenchymal transition. Its expression positively correlates with poor prognoses in several human and canine mammary cancers. The aim of this study was to characterise the immunohistochemical expression of LOX in MCT samples and compare it with histological grading and post-surgical survival. Twenty-six tumours were submitted to immunohistochemistry for LOX expression evaluation. All samples were positive for LOX, with variable percentages of cytoplasmic and nuclear positivity. Cytoplasmic positivity was significantly higher in high-grade MCTs (P = .0297). Our results indicate that high expression of cytoplasmic LOX in neoplastic mast cells is an indicator of poor prognosis for canine cutaneous MCTs. |
| Keywords: | dogs extracellular matrix immunohistochemistry lysyl oxidase mastocytoma prognosis |