| 耐酸性α-淀粉酶在家蚕生物反应器中的表达初探 |
| |
| 引用本文： | 王霞,张朝华,宋宪军,沈中元,黄深慧. 耐酸性α-淀粉酶在家蚕生物反应器中的表达初探[J]. 北方蚕业, 2014, 0(2): 10-12 |
| |
| 作者姓名： | 王霞 张朝华 宋宪军 沈中元 黄深慧 |
| |
| 作者单位： | 忖.广西壮族自治区蚕业技术推广总站,南宁,530007;中国农业科学院蚕业研究所,江苏镇江,212018 |
| |
| 摘 要： | 本文克隆了全基因3 906bp的热脂环酸杆菌的耐热酸性α-淀粉酶,此酶表达的蛋白分子量为140KD,有1 301个氨基酸。将此基因重组克隆到家蚕杆状病毒表达转移载体pVL1393中,使用一般方法获得重组病毒,并将该病毒感染家蚕,则酸性α-淀粉酶在家蚕中正常表达,对表达产物进行检测,此表达酶有淀粉酶活性。
|
| 关 键 词： | α-淀粉酶 重组病毒 克隆表达 |
Expression of Acid-stable Alpha-amylase in Silkworm Bioreactor |
| |
| Affiliation: | WANG Xia;ZHANG Chaohua;SONG Xianjun;SHEN Zhongyuan;HUANG Shenhui (1. Sericulture TechnoLogy Extension Station of Guangxl Zhuang Autonomous Region, Nanning 530007,China 2. The Sericulture Research Institute,Chinese Academy of Agricultural Science, Zhenjiang J iangsu 212018,China) |
| |
| Abstract: | The acid-stable alpha-amylase which was cloned from the Alicylobacillus acidocaldarius expressed by Bombyx mori bioreactor was taken to do the experiment in this study .The nucleotide sequence of Open Reading Frame of the gene acid-stableα-amylase was 3 906 bp ,encoding a polypeptide of 1 301 amino acids .The calculated molecular weight of this protein AMY was about 140KD .In this study ,recombinant virus was injected into Bom-byx mori using general method .The result is that AMY was expressed in silkworm larva .And the detection of the expression product showed amylase activity . |
| |
| Keywords: | α-amylase recombinant virus clone and expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
