| 6种寄主桃蛀果蛾遗传变异的RAPD分析 |
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| 引用本文： | 韩青梅,花 蕾.6种寄主桃蛀果蛾遗传变异的RAPD分析[J].西北农林科技大学学报(社会科学版),2001,29(3):91-94. |
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| 作者姓名： | 韩青梅 花 蕾 |
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| 作者单位： | 1. 西北农林科技大学畜牧兽医学院
2. 干旱半干旱地区农业研究中心, |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 用２５种随机引物对苹果、梨、杏、酸枣、枣、山楂６种寄主桃蛀果蛾基因组ＤＮＡ进行了随机扩增多态 ＤＮＡ（ＲＡＰＤ）分析，结果表明， ２５种随机引物中有 ２２种出现不同程度的 ＤＮＡ多态性标记，多态百分比为７１．９％．根据Ｎｅｉ氏片段共享度公式计算了６种寄主桃蛀果蛾两两配对间的遗传距离，６种寄主桃蛀果蛾的平均遗传距离为０．５４２ ６，其中梨桃蛀果蛾与酸枣桃蛀果蛾遗传距离最远为０．７０４，枣与酸枣遗传距离最近为０．４６２ ７，用ＵＰＧＭＡ法构建了６种寄主桃蛀果蛾的分子聚类图．
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| 关 键 词： | 桃蛀果蛾 RAPD-PCR 遗传变异 寄生 |
| 文章编号： | 1000-2782(2001)03-091-04 |
| 收稿时间： | 2000/7/11 0:00:00 |
| 修稿时间： | 2000年7月11日 |
RAPD-PCR analysis of genetic variation of Carposina sasakii Matsumura(Lepidoptera Carposinidae) in different host plants |
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| HAN Qing-mei,HUA Lei.RAPD-PCR analysis of genetic variation of Carposina sasakii Matsumura(Lepidoptera Carposinidae) in different host plants[J].Journal of Northwest Sci-Tech Univ of Agr and,2001,29(3):91-94. |
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| Authors: | HAN Qing-mei HUA Lei |
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| Institution: | 1 Collage of Animal Sciences and Veterinary Medicine; 2Center of Studying Aried and Semiaried Zone Agriculture Northwest Sci-Tech University of Agriculture and Forestry,Yangling ,Shaanxi 712100, China) |
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| Abstract: | In this research,RAPD-PCR was adopted to analyse the genomic DNA of Carposina sasakii Matsumura of 6 host plants (apple,pear,apricot,jujube,wild-jujube,hawthorn) with 25 random primers. As a result, 22 of the 25 random primers showed variable polymorphic marks with the polymorphic frequency of 71. 9%. Based on these results,we calculated the genetic distance of 6 populations. The average genetic distance of 6 populations was 0. 542 6,of which the most distant is between wild-jujube and pear,and the nearest is between jujube and wild-jujube. At the same time,the molecular dendrogram of the 6 populations was constructed with UPGMA method. |
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| Keywords: | Carposina sasakii Matsumura RAPD-PCR genetic variation host |
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