陆地棉与海岛棉两个末端氧化酶差异的比较

[目的]研究陆地棉与海岛棉2个末端氧化酶的差异,为陆海杂交育种材料选择提供理论依据.[方法]选用7份陆地棉、4份海岛棉为供试材料,比较了陆地棉和海岛棉不同发育阶段的抗坏血酸氧化酶(AAO)和多酚氧化酶(PPO)活性的变化的差异性.[结果]陆地棉和海岛棉2种末端氧化酶在整个发育进程中有相似的变化规律,AAO活性前期低后期高,且前期出现峰值;PPO活性表现为2个波峰,PPO前期活性高于后期,海岛棉PPO第1个波峰明显高于第2个波峰,陆地棉差异不大.[结论]海岛棉与陆地棉AAO、PPO活性在7月4日和8月6目的量存在差异,有可能为陆海杂交后代选育提供理论指导.Abstract：[Objective] This study was to reveal the differences in two terminal oxdiases,so as to provide theoretical basis for the selection of upland and sea-land hybrid materials.[Methods]Using 7 shares of upland cotton cuttivars and 4 shares of sea-land cultivars as experimental materials,the change differences in AAO and PPO activities during whole growth period of the materials tested were studied.[Results]Two terminal oxdiases both in upland and sea-land cotton cultivars assumed similar change laws during whole growth period.AAO activity assumed a low-high trend,and there is a peak at the early stage.PPO activity presented two peaks,and that at the early stage was higher than at the late stage;the first peak is obviously higher than the second for sea-land cultivars,and two peaks are not remarkably different for upland cultivars.[Conclusion] The differences in AAO and PPO activities of sea-land and upland cotton cultivars observed on Jul.4th and Aug.6th,may provide guidance for the selection of upland and sea-land hybrids.

Comparison of the Activity Differences in Two Terminal Oxidases between Upland Cotton and Sea-island Cotton

Sea-land cotton Upland cotton Growth stage Terminal oxdase