| 柑橘黄龙病病原DNA提取方法比较 |
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| 引用本文： | 王春梅,张秋明,罗齐克,王红.柑橘黄龙病病原DNA提取方法比较[J].西南农业学报,2009,22(5). |
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| 作者姓名： | 王春梅 张秋明 罗齐克 王红 |
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| 作者单位： | 1. 湖南岳阳职业技术学院生物科技系,湖南,岳阳,414000
2. 湖南农业大学园艺园林学院,湖南,长沙,410128 |
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| 基金项目： | 岳阳职业技术学院基金项目 |
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| 摘 要： | 柑橘植物组织中富含多糖、酚类、单宁、色素等物质,它们的存在严重干扰了柑橘黄龙病PCR检测的准确性.因此有必要筛选出一种能得到纯度高、结构完整的柑橘总DNA(含黄龙病病原DNA),且操作简便的提取方法.本研究对比试验了3种柑橘总DNA提取方法,方法1采用Sandrine等人的提取法;方法2采用CTAB法;方法3采用试剂盒法.实验研究表明,方法2提取柑橘黄龙病病原DNA的PCR检测准确率最高.
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| 关 键 词： | 柑橘黄龙病 柑橘总DNA |
Comparicon of Extraction Methods of DNA from Citrus Huanglongbing Pathogen |
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| WANG Chun-mei,ZHANG Qiu-ming,LUO Qi-ke,WANG Hong.Comparicon of Extraction Methods of DNA from Citrus Huanglongbing Pathogen[J].Southwest China Journal of Agricultural Sciences,2009,22(5). |
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| Authors: | WANG Chun-mei ZHANG Qiu-ming LUO Qi-ke WANG Hong |
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| Abstract: | The PCR test of citrus Hulongbing was affected by some substance in citrus including polysaccharides,Phenolic,tannic acid,pigment and so on.It was necessary to establish a simple method to extract high purity and integrated construction citrus DNA. Three method were used in this experiment. There were Sandrine method, CTAB method,and Reagent box method. The results showed in these methods, CTAB was the best one in the three. |
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| Keywords: | PCR |
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