黑龙江省松茸生长地土壤微生物量及土壤酶活性研究

以黑龙江省松茸生长地土壤为研究对象,对不同海拔高度的土壤理化性质、酶活性和微生物量进行测定.结果表明,不同海拔高度土壤基本理化性质间存在一定差异.多酚氧化酶、纤维素酶和酸性磷酸酶活性变化范围分别为0.77～1.23、0.62～1.39、1.52～2.58 mg/g,均随海拔升高而显著下降;过氧化氢酶活性在山上部最高(9.37mL/g)、山顶部最低(5.85 mL/g),且不同海拔间差异显著;脲酶活性在山下部(0.96 mg/g)显著高于其他样地,山中部和山上部显著高于山顶部(0.51 mg/g);蛋白酶活性在山中部(3.07 mg/g)和山上部(2.98 mg/g)显著高于其他样地.土壤微生物量碳和微生物量氮的变化表现出相同的规律,均为山上部＞山中部＞山下部＞山顶部.相关性分析表明,土壤酶活性和微生物量与土壤有机质、全氮(除纤维素酶)和全磷(除过氧化氢酶)均达到显著(P＜0.05)或极显著(P＜0.01)正相关关系,而与土壤含水量、pH值和全钾相关性较差.

Study on microbial biomass and enzyme activity in Tricholoma matsutake growing soil in Heilongjiang province

This paper took Tricholoma matsutake growing soil in Heilongjiang province as the research object,measured the physical and chemical properties, enzyme activity and microbial biomass of soil in different altitude respectively. The results showed that there existed certain differences with the basic physical and chemical properties of soil in different altitude. The activity of polyphenol oxidase, cellulose and acid phosphatase, ranged from 0.77-1.23, 0.62-1.39 and 1.52-2.58 mg/g, respectively, declined significantly with altitude; the activity of hydrogen peroxide enzyme in the upper part of the mountain was the highest (9.37 mL/g) but the lowest in the top part (5.85 mL/g), and it had significant difference in different altitude; the activity of urease in the lower part of the mountain (0.96 mg/g) was significantly higherthan that in other sample plots, and it was significantly higher in the middle and upper part of the mountain than that inthe top part (0.51 mg/g); the activity of protease in the middle (3.07 mg/g) and upper part (2.98 mg/g) of the mountain was significantly higher than that in other sample plots. The change of soil microbial biomass carbon and microbial biomass nitrogen showed the same regularity: the data in the upper part of the mountain was over that in the middle part, then over that in the lower part and was greater than that in the top part. Correlation analysis showed that soil enzyme activity and microbial biomass had significant (P<0.05) or very significant (P<0.01) positive correlation with soil organic matter, total nitrogen (except cellulose enzyme) and total phosphorus (except catalase), but had poor correlation with soil water content, pH value and total potassium.