| 长春花种子油脂肪酸成分的GC-MS分析 |
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| 引用本文： | 牛卉颖,杨磊,张琳,李晓薇,刘玉,祖元刚.长春花种子油脂肪酸成分的GC-MS分析[J].浙江林学院学报,2008,25(2):259-261. |
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| 作者姓名： | 牛卉颖 杨磊 张琳 李晓薇 刘玉 祖元刚 |
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| 作者单位： | 东北林业大学,森林植物生态学教育部重点实验室,黑龙江,哈尔滨150040 |
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| 基金项目： | 国家科技支撑计划
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国家林业局重点科学技术研究资助项目 |
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| 摘 要： | 旨在建立长春花Catharanthus roseus种子脂肪酸成分的检测方法。采用正己烷提取长春花种子油，然后将油样甲酯化，以气相色谱-质谱（GC-MS）联用技术测定其中的组分。从分离出的9个峰中确认了5种组分，采用峰面积归一法计算各组分的相对百分含量，所鉴定的组分占总峰面积的99.91％。主要脂肪酸油酸（C19H36O2）、亚油酸（C19H34O2）、棕榈酸（C17H34O2）和花生酸（C21H42O2）的相对含量分别是73．92％，16．93％，5．58％和3．40％。表1参12。
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| 关 键 词： | 植物学 长春花 种子油 脂肪酸 气相色谱-质谱法 |
| 文章编号： | 1000-5692(2008)02-0259-03 |
| 修稿时间： | 2007年4月22日 |
Analysis of the fatty acid composition in Catharanthus roseus seed oil by gas chromatography-mass spectrometry |
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| NIU Hui-ying,YANG Lei,ZHANG Lin,LI Xiao-wei,LIU Yu,ZU Yuan-gang.Analysis of the fatty acid composition in Catharanthus roseus seed oil by gas chromatography-mass spectrometry[J].Journal of Zhejiang Forestry College,2008,25(2):259-261. |
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| Authors: | NIU Hui-ying YANG Lei ZHANG Lin LI Xiao-wei LIU Yu ZU Yuan-gang |
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| Institution: | (Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China) |
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| Abstract: | Catharanthus roseus seed oil was extracted with n-Hexane, then esterified with potassium hydroxide and methanol. After esterification, the oil was analyzed by gas chromatography-mass spectrum （GC-MS）. 9 peaks were separated and 5 peaks were identified, the compounds were quantitatively determined by normalization method, and the identified compounds accounted for 99.91% of total GC peak areas. The contents of the main fatty acids, namely, oleic acid, linoleic acid, palmitic acid, arachidic acid relative contents were respectively 73.92% , 16.93% , 5.58% and 3.40%. Ch, 1 tab. 12 ref. ] |
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| Keywords: | botany Catharanthus roseus seed oil fatty acid GC-MS |
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